Reagents for measuring lipase activity

Abstract

A reagent for measuring a lipase activity comprised of a higher fatty acid ester having the general formula ##STR1## wherein R.sup.1 is a straight chain alkyl group; R.sup.2 is hydrogen or a halogen atom; R.sup.3 and R.sup.4 are independently a nitro group, an alkylsulfonyl group, a substituted alkylsulfonyl group or a trifluoromethyl group; G is an --SO.sub.3 M group or a group having at least one --SO.sub.3 M where M represents sodium or potassium, or, when R.sup.4 has a sulfonic acid group, may be a hydrogen atom; and Q is hydrogen, a halogen atom, an alkyl group, an alkoxy-substituted alkyl group, an alkoxy group, an alkoxy-substituted alkoxy group, a sulfonamido group, a sulfamoyl group, a carbonamido group, a carbamoyl group, or an alkylsulfonyl group. The reagent is capable of releasing a dye on enzymatic hydrolysis by the action of lipase. Because of the released dye has a light absorption zone within the longer wavelength region, it is not substantially disturbed by colored matter contained within blood making it possible to obtain excellent operability and reproducibility in the measurement of lipase activity within blood.

Dependencies are inferred from the claim text.