Synthesis of nicotinamide cofactors
AssigneeMassachusetts Institute of Technology
InventorsGeorge Whitesides, Davis R. Walt
Life of this patent
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Abstract
Nicotinamide cofactors are prepared in a process of reacting ribose -5- phosphate with a basic material selected from the group consisting of ammonia, primary and secondary amines in a polar non-aqueous solvent, reacting the resultant 1-ribosylamine -5- phosphate with a pyridinium salt and reacting the resultant nicotinamide mononucleotide with adenosine triphosphate in the presence of nicotinamide adenine dinucleotide pyrophosphorase to produce nicotinamide adenine dinucleotide which can be used directly in crude form without further purification in co-factor - requiring enzymatic reactions. The nicotinamide adenine dinucleotide pyrophosphorase may be immobilized on a solid support.
Claims11
A method of preparing nicotinamide mononucleotide comprising the steps of
- (a) reacting ribose-5-phosphate with a basic material selected from the group of ammonia, primary and secondary amines and mixtures thereof in a polar, non-aqueous solvent solution;
- and
- (b) reacting the resulting product with a pyridinium salt having the following formula:
- ##STR2## where R is a nitroaromatic group, R' is a carbamoyl, alkyl, acetyl or alkoxycarbonyl group and X is a halide or other anion for charge balance.
A method of preparing nicotinamide adenine dinucleotide comprising the steps of:
- (a) reacting ribose-5-phosphates with a basic material selected from the group of ammonia, primary and secondary amines and mixtures thereof while in a polar, non-aqueous solution;
- (b) reacting the resulting product with a pyridinium salt having the following formula:
- ##STR3## where R is a nitroaromatic group, R' is a carbamoyl, alkyl, acetyl or alkoxycarbonyl group and X is a halide or other anion for charge balance;
- and
- (c) reacting the second resulting product with adenosine triphosphate in the presence of nicotinamide adenine dinucleotide pyrophosphorase.
Dependencies are inferred from the claim text.