Substrates and method for determining enzymes
OwnerRobert E. Smith, Eugene R. Bissellinventors
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Abstract
A method is disclosed for determining the presence of an enzyme in a biological fluid, which includes the steps of contacting the fluid with a synthetic chromogenic substrate, which is an amino acid derivative of 7-amino-4-trifluoromethylcoumarin; incubating the substrate-containing fluid to effect enzymatic hydrolysis; and fluorometrically determining the presence of the free 7-amino-4-trifluoromethylcoumarin chromophore in the hydrolyzate.
Claims9
A proteolytic enzyme substrate of the formula ##STR6## wherein R is an amino acid, a peptide, an amino acid which has been reacted with a blocking group, or a peptide, the terminal amino acid of which has been reacted with a blocking group;
- and R is releaseable therefrom by enzymatic hydrolysis to form a measureable amount of 7-amino-4-trifluoromethylcoumarin.
Dependencies are inferred from the claim text.