Back to results
US 4,407,956 AGranted
Cloned cauliflower mosaic virus DNA as a plant vehicle
AssigneeThe Regents of the University of California
InventorStephen H. Howell
Life of this patent
Estimating the term…
Loading patent…
Loading patent…
AssigneeThe Regents of the University of California
InventorStephen H. Howell
Estimating the term…
Novel methods and compositions are provided for preparing vectors for the introduction of DNA into plant cells for transcription and expression of the DNA. Particularly, cauliflower mosaic virus DNA is inserted into a bacterial cloning vehicle to provide a recombinant plasmid for cloning in a microorganism. The resulting cloned plasmid is genetically manipulated to introduce exogenous or heterologous DNA. Conveniently, linkers can be inserted which provide for a unique restriction site for insertion of exogenous or heterologous DNA. At each stage the modified plasmid may be cloned to provide for relatively large amounts of material for modification and isolation. Besides insertions, deletions may be made, removing non-essential portions of the virus. After completion of the viral modifications, the CaMV is excised from the hybrid plasmid by restriction enzyme cleavage and may be used for systemic infection of plants.
A method for modifying Cauliflower mosaic viral DNA while maintaining the capability of movement, said movement capability comprising replication and systemic infection, which comprises:
Recombinant Cauliflower mosaic virus capable of propagation and movement, said movement comprising replication and systemic infection, said virus or a parent thereof having received in vitro an insertion of foreign DNA at the intergenic region between reading frames VI and I, a site non-essential to such movement.
Dependencies are inferred from the claim text.