Silver stains for protein in gels
AssigneeThe United States of America as represented by the Department of Health and Human Services
InventorCarl R. Merril
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Abstract
A silver stain method for polypeptides in gels comprising the sequential steps of photo-reversing the polypeptide-gel by treatment with an oxidizing agent, forming a latent stain image by treating the polypeptide-gel with a photosensitive salt, and developing the stain image by treating the polypeptide-gel with a reducing agent.
Claims16
A silver stain method for polypeptides fixed in gels comprising the sequential steps without substantial non-ambient illumination of
- photo-reversing the polypeptide-gel by treatment with an oxidizing agent,
- forming a latent stain image by treating the polypeptide-gel with a photosensitive salt, and
- developing the stain image by treating the polypeptide-gel with a reducing agent.
A silver stain method for polypeptides comprising the sequential steps without substantial non-ambient illumination of
- mixing the polypeptides with an aqueous solution of a fixing agent to form a polypeptide precipitate,
- filtering the mixture with filter paper,
- photoreversing the polypeptide precipitate by treatment with an oxidizing agent,
- forming a latent stain image by treatment of the polypeptide precipitate with a photosensitive salt, and
- developing the stain image by treatment of the polypeptide precipitate with a reducing agent.
A silver stain method for fixed tissue comprising the sequential steps without substantial non-ambient illumination of
- photoreversing a fixed tissue specimen by treatment with an oxidizing agent,
- forming a latent image by treating the tissue with a photosensitive salt, and
- developing the stain image by treating the tissue with a reducing agent.
A silver stain method for polypeptides fixed in gels comprising the sequential steps of
- photo-reversing the polypeptide-gel by treatment with an oxidizing agent selected from the group consisting of dichromates, potassium permanganate, molecular oxygen, iodine, and quinone,
- forming a latent stain image by treating the polypeptide-gel with a photosensitive salt,
- subjecting the polypeptide-gel to actuating irradiation by exposure to relatively intense uniform light during the first minutes of treatment with the photosensitive salt, and
- developing the stain image by treating the polypeptide-gel with a reducing agent.
Dependencies are inferred from the claim text.