# US 4,400,472 A · Novel rabies virus strain and a process for its preparation

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- Number: US 4,400,472 A
- Document ID: US4400472A
- Status: Granted
- Kind: A
- Application number: 06/310,447
- Filed: 1981-10-13
- Granted: 1983-08-23
- Earliest claimed: 1977-01-26
- Assignees: Gist-Brocades N.V.
- Inventors: Gosse Bijlenga
- Web page: https://patentgrep.com/patent/US4400472A

## CPC

- A61K 39/205: Rhabdoviridae, e.g. rabies virus
- A61K 39/12: Viral antigens
- A61K 2039/5252: {inactivated (killed)}
- A61K 2039/5254: {avirulent or attenuated}
- A61K 2039/542: {oral/gastrointestinal}
- A61K 2039/552: {Veterinary vaccine}
- C12N 2760/20134: Use of virus or viral component as vaccine, e.g. live-attenuated or inactivated virus, VLP, viral protein
- A61K: PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES (devices or methods specially adapted for bringing pharmaceutical products into particular physical or administering forms A61J3/00; chemical aspects of, or use of materials for deodorisation of air, for disinfection or sterilisation, or for bandages, dressings, absorbent pads or surgical articles A61L; soap compositions C11D)
- C12N: MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA (microbiological testing media C12Q1/00)

## Abstract

A novel rabies virus strain No. 675 deposited in the Czechoslovak National Collection of Type Cultures of the Institute of Hygiene and Epidemiology in Prague under number CNCTO AO 4/77, novel living or inactivated cell culture vaccines derived therefrom, a process for the isolation of the virus strain as well as a process for the preparation of the said vaccines and novel method of immunizing warm-blooded animals against rabies.

## Claims

### Claim 1

A rabies virus strain exhibiting reproducibly significant cytopathic effect on in vitro cell cultures of primary or secondary SPF chicken embryo fibroblasts of strain No. 675 deposited with the Czechoslovak National Collection of Type Culture of the Institute of Hygiene and Epidemiology in Prague deposited under CNCTC AO 4/77.

### Claim 2 (depends on 1)

A process for the preparation of live rabies virus of claim 1 exhibiting reproducibly significant cytopathic effect of strain No. 675 deposited with the Czechoslovak National Collection of Type Culture of the Institute of Hygiene and Epidemiology in Prague comprising propagating HEP Flury virus in primary or secondary SPF chicken embryo fibroblasts, or in rabies susceptible cell lines and dipolid cell strains isolating a plaque therefrom which is distinguishable from the parent strain by its very pronounced cytopathic effect on the infected cell by use of a plaque assay method and a plaquing cell suspension method, and effecting three clone purifications of the obtained plaque to obtain the desired homogeneous and pure virus population.

### Claim 3

Rabies virus strain novel species No. 675 deposited with the Czechoslovak National Collection of Type Cultures of the Institute of Hygiene and Epidemiology in Prague under CNCTC AO 4/77 distinguishable from the original HEP-Flury strain by
(a) a very pronounced cytopathic effect
(b) a shorter primary virus cycle
(c) clearer and larger plaques
(d) enhanced attaching capacity to the surface of cells
(e) enhanced induction of interferon
(f) higher virus-titres in culture
(g) greater homogenicity of the strain
(h) greatly reduced pathogenicity to a variety of mammals, and its mutants and variants exhibiting the above mentioned characteristics.