# US 4,399,362 A · Liquid handling apparatus

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- Number: US 4,399,362 A
- Document ID: US4399362A
- Status: Granted
- Kind: A
- Application number: 06/239,025
- Filed: 1981-02-27
- Granted: 1983-08-16
- Earliest claimed: 1981-02-27
- Assignees: Instrumentation Laboratory Company
- Inventors: Alan D. Cormier; John D. Czaban; Thomas J. Schillinger; Kenneth D. Legg; Thomas F. Kelley
- Web page: https://patentgrep.com/patent/US4399362A

## CPC

- G01N 35/08: using a stream of discrete samples flowing along a tube system, e.g. flow injection analysis
- G01N: INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES (measuring or testing apparatus or processes other than immunoassay, involving enzymes or microorganisms C12M, C12Q)

## Abstract

A clinical analyzer system flows a small volume (less than 200 microliters) of liquid sample to be analyzed from an inlet port past a sensor array to a series of analysis chambers. The sensor array includes two optical sensor units that are spaced a fixed distance apart, and each sensor unit transmits a beam of infrared radiation through the flow tube for sensing by a detector. Each detector output has a low value when blood is in the flow tube, an intermediate value when air is in the flow tube, and a high value when a clear liquid is in the flow tube. Control logic senses changes in the outputs of the detectors and accurately indicates gas-liquid interface transitions. The noninvasive system accurately locates leading and trailing edges of samples of biological fluids to be analyzed, monitors the integrity of those samples, and distinguishes between types of sample liquids processed by the analyzer.

## Claims

### Claim 1

A system for analyzing a biological fluid comprising
an analysis region,
a measurement system connected in sensing relation to said analysis region,
a sample flow path connected between a sample inlet port and said analysis region,
a pump for flowing sample to be analyzed through said sample flow path to said analysis region,
a gas-liquid interface sensor in said flow path, said sensor having a first output when blood is in said flow path, a second output of magnitude different from the magnitude of said first output when serum is in said flow path, and a third output of magnitude intermediate the magnitudes of said first and second outputs when air is in said flow path, and
control means for operating said pump at a first rate and responsive to said interface sensor for positioning the leading edge of a sample of fluid to be analyzed at a predetermined location in said sample flow path and then operating said pump at a second rate different from said first rate determined by the type of fluid detected by said sensor for positioning the sample in said analysis region with the trailing edge of the sample at a second predetermined location in said sample flow path.

### Claim 2 (depends on 1)

The system of claim 1 wherein said sensor is arranged to transmit a beam of infrared radiation through said flow path for sensing by a detector on the opposite side of said flow path, and said control means includes threshold logic responsive to changes in the output of said sensor for indicating the nature of the fluid in said flow path.

### Claim 3 (depends on 1)

The system of claim 1 and further including logic responsive to said sensor outputs that includes a plurality of threshold detector circuits.

### Claim 4 (depends on 1)

The system of claim 1 and further including a second gas-liquid interface sensor in said flow path,
said two gas liquid interface sensor units being spaced a predetermined distance apart along said flow path for sensing the transit time of a gas-liquid interface between said sensor units,
storage means for storing said transit time value as sensed by said interface sensor units, and
said control means is responsive to said stored transit time value for operating said pump for an interval of time that is a predetermined multiple of said stored transit time value for positioning a predetermined volume of liquid to be analyzed in said analysis region.

### Claim 5 (depends on 4)

The system of claim 4 wherein each said sensor unit includes a radiation source for directing a beam of infrared radiation through said flow path for sensing by a detector on the opposite side of said flow path.

### Claim 6 (depends on 1)

The system of claim 1 wherein said pump is driven by a stepping motor, and further including means responsive to the type of liquid in said flow path as sensed by said sensor for selecting a pump stepping pulse rate.

### Claim 7 (depends on 6)

The system of claim 6 and further including a second pump for pumping flush fluid through said sample flow path in a backflush direction and means responsive to said sensor for indicating depletion of flush fluid.

### Claim 8 (depends on 1)

The system of claim 1 wherein said measurement system includes an electrochemical electrode with an ion selective membrane that is arranged for exposure to sample in said sample flow path.