# US 4,399,274 A · Isolation of non-ionic lipophilic materials on macroreticular polymeric absorbents

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- Number: US 4,399,274 A
- Document ID: US4399274A
- Status: Granted
- Kind: A
- Application number: 06/279,633
- Filed: 1981-07-02
- Granted: 1983-08-16
- Earliest claimed: 1981-07-02
- Assignees: Merck & Co., Inc.
- Inventors: Robert T. Goegelman; Laszlo R. Treiber
- Web page: https://patentgrep.com/patent/US4399274A

## CPC

- C07H 19/01: sharing oxygen
- C07H: SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS (derivatives of aldonic or saccharic acids C07C, C07D; aldonic acids, saccharic acids C07C59/105, C07C59/285; cyanohydrins C07C255/16; glycals C07D; compounds of unknown constitution C07G; polysaccharides, derivatives thereof C08B; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification C12N15/00; sugar industry C13)

## Abstract

Non-ionic, lipophilic substances are isolated from aqueous or aqueous-organic solution by treatment with a macroreticular polymeric absorbent followed by elution with additional or a different organic solvent.

## Claims

### Claim 1

A process for isolating or separating avermectin compounds from the aqueous fermentation broth that produced such compounds, which comprises:
preparing a mycelial extract by adding acetone to the fermentation broth to from 40 to 60% by volume of the original broth to form a mycelial extract:
treating the aqueous-acetone mycelial extract with an insoluble synthetic resin which is an addition copolymer having a cross linked structure to selectively absorb the avermectin compounds into the resin: and
eluting the resin with an aqueous acetone mixture in excess of 60% acetone to remove the avermectin compounds from the resin.