# US 4,399,229 A · Rapid radioimmunoassay product and method of making and using same

> Agents: this data is available as JSON from the patentgrep API and MCP server — see https://patentgrep.com/llms.txt

- Number: US 4,399,229 A
- Document ID: US4399229A
- Status: Granted
- Kind: A
- Application number: 06/139,770
- Filed: 1980-04-14
- Granted: 1983-08-16
- Earliest claimed: 1980-04-14
- Assignees: Immutron, Inc.
- Inventors: Arden A. Kelton; Michael L. Bell; Roy A. Chung
- Web page: https://patentgrep.com/patent/US4399229A

## CPC

- G01N 33/554: the carrier being a biological cell or cell fragment, e.g. bacteria, yeast cells
- Y10S 436/804: Radioisotope, e.g. radioimmunoassay
- Y10S 436/82: Hepatitis associated antigens and antibodies
- Y10S 436/828: Protein A
- G01N: INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES (measuring or testing apparatus or processes other than immunoassay, involving enzymes or microorganisms C12M, C12Q)
- Y10S: TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS

## Abstract

A stabilized radioimmunoassay product consisting of an antibody protein-bound to the cell wall of a selected bacterium whereby the antibody is irreversibly bound to the protein and remains specific for the antigen against which it was developed. The radioimmunoassay product is also characterized by the fact that the resultant complete product includes a phase of serum protein treatment to isolate the protein sites or other sites of nonspecific reactivity with the labelled antigen such that the non-specific binding of labelled antigen is significantly reduced. Also within the contemplation of the invention is the method of manufacturing the radioimmunoassay product which includes the initial phase of protein binding of the antibody to the selected bacterium and the subsequent serum treatment to isolate previously unutilized protein sites such that when said sites are subsequently exposed to labelled antigen it will be rejected. Further within the contemplation of the invention is the method of utilizing the aforesaid product to totally eliminate the necessity for the customary incubation stages necessitated by prior art radioimmunoassay techniques.

## Claims

### Claim 1

In a composite for conducting an immunoassay the combination of: a filter for retaining a bacterium to which an antibody is bound; and said bacterium being entrapped in said filter whereby, when reagents are caused to flow through said filter, said reagents contact said bacterium to facilitate the reaction of said reagents with said antibody.

### Claim 2 (depends on 1)

The composite of claim 1 in which said bacterium is deposited out of suspension into said filter.

### Claim 3 (depends on 1)

The composite of claim 1 in which said antibody is irreversibly bound to specific receptors of said bacterium.

### Claim 4

In a method of conducting an immunoassay, the steps of: depositing an antibody bound bacterium in a filter to entrap the same therein; flowing a reagent through said filter to react with said antibody; and detecting the results of the reaction.