# US 4,399,220 A · Stabilizing lactobacilli

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- Number: US 4,399,220 A
- Document ID: US4399220A
- Status: Granted
- Kind: A
- Application number: 06/016,085
- Filed: 1979-02-28
- Granted: 1983-08-16
- Earliest claimed: 1978-03-16
- Assignees: Societe d'Assistance Technique pour Produits Nestle S.A.
- Inventors: Martin B. Smiley
- Web page: https://patentgrep.com/patent/US4399220A

## CPC

- C12P 7/56: Lactic acid
- A23C 9/1234: {characterised by using a Lactobacillus sp. other than Lactobacillus Bulgaricus, including Bificlobacterium sp.}
- C12N 1/20: Bacteria; Culture media therefor
- C12N 15/00: Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor (mutants or genetically engineered microorganisms, per se C12N1/00, C12N5/00, C12N7/00; new plants per se A01H; plant reproduction by tissue culture techniques A01H4/00; new animals per se A01K67/00; use of medicinal preparations containing genetic material which is inserted into cells of the living body to treat genetic diseases, gene therapy A61K48/00)
- C12P: FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE {(brewing of beer C12C; producing vinegar C12J; producing specific peptides or proteins C07K; producing enzymes C12N9/00; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification C12N15/00; measuring or testing processes involving enzymes or microorganisms C12Q; measuring or testing processes involving nucleic acid amplification reactions C12Q1/6844; fermentation processes to form a food composition, A21 or A23; compounds in general, see the relevant compound class, e.g. C01, C07)}
- A23C: DAIRY PRODUCTS, e.g. MILK, BUTTER OR CHEESE; MILK OR CHEESE SUBSTITUTES; PREPARATION THEREOF (preservation thereof A23B11/00)
- C12N: MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA (microbiological testing media C12Q1/00)

## Abstract

A process for producing a stable culture of a microorganism of the genus Lactobacillus containing at least one extrachromosomal genetic element which, on the one hand, stimulates its ability to produce lactic acid from lactose and which, on the other hand, controls its ability to metabolize N-acetyl-D-glucosamine, which comprises cultivating the microorganism in a nutrient medium of which the principal source of assimilable carbon is N-acetyl-D-glucosamine.

## Claims

### Claim 1

A process for producing a stable culture of a microorganism of the genus Lactobacillus containing at least one extrachromosomal genetic element which, on the one hand, stimulates its ability to produce lactic acid from lactose and which, on the other hand controls its ability to metabolise N-acetyl-D-glucosamine, which comprises cultivating the microorganism in a nutrient medium of which the principal source of assimilable carbon is N-acetyl-D-glucosamine.

### Claim 2 (depends on 1)

A process as claimed in claim 1, wherein the microorganism is Lactobacillus helveticus of the subspecies, jugurti obtainable under No. S 36-2 from the Institute of Agronomic Microbiology of the University of Bologna in Italy.