Method and reagent for the determination of glycerin
AssigneeBoehringer Mannheim GmbH
InventorsHelmgard Gauhl, Hans Seidel, Gunter Lang, Albert Roder, Joachim Ziegenhorn
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Abstract
For the determination of glycerin by oxidation with oxygen in the presence of glycerinoxidase and measurement of the oxygen consumption or of the H.sub.2 O.sub.2 formation, a glycerinoxidase from Aspergillus spec. DSM 1729 is used. A reagent suitable for this method consists of glycerinoxidase from Aspergillus spec. DSM 1729 and a system for the determination of H.sub.2 O.sub.2 and contains additionally, if desired, an agent for the saponification of esterified glycerin.
Claims16
Method for the determination of glycerin by oxidation with oxygen in the presence of glycerinoxidase and measurement of the oxygen consumption or of the formation of H.sub.2 O.sub.2, characterized in that a glycerinoxidase from Aspergillus spec. DSM 1729 is used.
Reagent for the determination of glycerin, characterized in that it consists of glycerinoxidase from Aspergillus spec. DSM 1729 and a system for the determination of H.sub.2 O.sub.2.
Method of producing glycerinoxidase from Aspergillus spec. DSM 1729, characterized in that Aspergillus spec. DSM 1729 is cultured in a suitable nutrient medium and the glycerinoxidase is obtained from the cell mass or from the culture medium.
Glycerinoxidase from Aspergillus spec. DSM 1729 having a molecular weight of about 90000, a specific activity of 2000 to 8000 U/mg, which is inhibited by SH reagents and not inhibited by copper sulfate and lead acetate.
Dependencies are inferred from the claim text.