# US 4,399,217 A · Process and a device for the determination of serum lipoproteins > Agents: this data is available as JSON from the patentgrep API and MCP server — see https://patentgrep.com/llms.txt - Number: US 4,399,217 A - Document ID: US4399217A - Status: Granted - Kind: A - Application number: 06/224,538 - Filed: 1980-12-10 - Granted: 1983-08-16 - Earliest claimed: 1979-05-02 - Assignees: Laboratoires Goella - Inventors: Leif T. Holmquist; Lars A. Carlson - Web page: https://patentgrep.com/patent/US4399217A ## CPC - G01N 33/92: involving lipids, e.g. cholesterol {, lipoproteins, or their receptors (steroid hormones G01N33/743)} - Y10S 435/97: Test strip or test slide - Y10S 435/975: Kit - Y10S 436/808: Automated or kit - G01N: INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES (measuring or testing apparatus or processes other than immunoassay, involving enzymes or microorganisms C12M, C12Q) - Y10S: TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS ## Abstract The invention relates to a process for the determination of serum lipoproteins by an immuno-enzymatic method. The process of the invention consists in reacting a specific antibody of the apoprotein whose amount is to be measured, fixed on a support, with, on the one hand, the compound for coupling the corresponding lipoprotein with an enzyme and, on the other hand, the analyzed sample. The amount of enzyme fixed is then an inverse function of the apoprotein content of the sample. The support with the products which are fixed thereto are separated from the rest of the reagents and the activity of the enzyme of the support is measured which is compared with standard measurements. The process is useful for serum analyses, particularly for early detection of cardiovascular disease risks. ## Claims ### Claim 1 Process for the determination of serum apoproteins consisting in reacting, on the one hand, a given amount of a specific antibody of the apoprotein whose amount is to be determined, said antibody being fixed on an inert solid support, and, on the other hand, the serum sample to be analyzed and a given amount of an enzyme-lipoprotein coupling compound, corresponding to the apoprotein whose amount is to be determined, after completion of the antibody-lipoprotein reaction separating the support from the non-fixed reagents, and then measuring the enzymatic activity of the complex retained on the support, and wherein said coupling compound is formed by fixation of an enzyme on a fraction of serum lipoprotein containing several distinct apoproteins. ### Claim 2 (depends on 1) The process of claim 1 wherein the apoproteins C.sub.1, C.sub.2, C.sub.3 and E are determined using VLDL-conjugate as the enzyme-lipoprotein coupling compound. ### Claim 3 (depends on 1) Process according to claim 1, wherein said lipoprotein fraction is either the VLDL fraction, or the LDL fraction, or the HDL fraction, such as defined in ultracentrifugation separation methods. ### Claim 4 (depends on 1) Process according to claim 1, wherein said enzyme-lipoprotein coupling compound results from coupling by means of glutaraldehyde or by formation of a Schiff base. ### Claim 5 (depends on 1) Process according to claim 1, wherein aid enzyme of the enzyme-lipoprotein compound is alkaline-phosphatase, peroxydase or galactose oxydase. ### Claim 6 (depends on 1) Process according to claim 1, wherein said antibody is fixed to the solid support in a covalent way. ### Claim 7 (depends on 1) Process according to claim 1, wherein said antibody is fixed to the support by adsorption. ### Claim 8 (depends on 7) Process according to claim 7, wherein said solid support is coated with a film of an adsorbing material. ### Claim 9 (depends on 8) Process according to claim 8, wherein said adsorption material is a cellulose or a cellulose derivative. ### Claim 10 (depends on 1) Process according to claim 1, wherein said support for the antibody is the vessel in which the analysis is effected. ### Claim 11 (depends on 10) Process according to claim 10, wherein after said antibody-lipoprotein reaction, the vessel is emptied and washed, and the reagents for measuring the enzymatic activity are introduced therein, the measurement being effected in the vessel itself. ### Claim 12 A kit assembly for determination of serum apoproteins consisting essentially of at least one vessel containing at least one immobilized specific antibody of a serum apoprotein and the corresponding lipoprotein(s)-enzyme. ### Claim 13 (depends on 12) The assembly of claim 12 wherein said vessel is an hemolysis tube, a spectrophotometric tank, or a microfiltration plate. ### Claim 14 (depends on 13) The assembly of claim 13 wherein said antibody is fixed on the wall of the vessel itself. ### Claim 15 (depends on 12) The assembly of claim 12 further including at least one standard lipoprotein. ### Claim 16 (depends on 12) The assembly of claim 12 further including reagents required for measurement of enzymatic activity. ### Claim 17 (depends on 12) The assembly of claim 12 further including measuring and washing media solutions.