# US 4,398,382 A · Method for washing and coating as well as for dosage of EIA-rings and a device for carrying out the method

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- Number: US 4,398,382 A
- Document ID: US4398382A
- Status: Granted
- Kind: A
- Application number: 06/252,180
- Filed: 1981-04-08
- Granted: 1983-08-16
- Earliest claimed: 1980-04-28
- Assignees: Kommandiittiyhtio Finnpipette Osmo A. Suovaniemi
- Inventors: Osmo A. Suovaniemi; Jukka Tervamaki; Jukka Suni; Paul Partanen
- Web page: https://patentgrep.com/patent/US4398382A

## CPC

- C07K 16/26: against hormones {; against hormone releasing or inhibiting factors}
- G01N 33/531: Production of immunochemical test materials
- G01N 33/54366: {Apparatus specially adapted for solid-phase testing}
- G01N 33/577: involving monoclonal antibodies {binding reaction mechanisms characterised by the use of monoclonal antibodies (G01N33/5302 - G01N33/576 take precedence)}
- G01N 33/743: {Steroid hormones}
- A61K 38/00: Medicinal preparations containing peptides (peptides containing beta-lactam rings A61K31/00; cyclic dipeptides not having in their molecule any other peptide link than those which form their ring, e.g. piperazine-2,5-diones, A61K31/00; ergot alkaloids of the cyclic peptide type A61K31/48; containing macromolecular compounds having statistically distributed amino acid units A61K31/74; medicinal preparations containing antigens or antibodies A61K39/00; medicinal preparations characterised by the non-active ingredients, e.g. peptides as drug carriers, A61K47/00)
- A61K 2039/6093: {Synthetic polymers, e.g. polyethyleneglycol [PEG], Polymers or copolymers of (D) glutamate and (D) lysine}
- Y10S 436/804: Radioisotope, e.g. radioimmunoassay
- Y10S 436/807: Apparatus included in process claim, e.g. physical support structures
- Y10S 436/815: Test for named compound or class of compounds
- Y10S 436/817: Steroids or hormones
- Y10S 436/818: Human chorionic gonadotropin
- Y10S 436/823: Immunogenic carrier or carrier per se
- Y10S 930/29: Polyamino acid or polypeptide with an uninterrupted series of peptide repeating units
- C07K: PEPTIDES (peptides in foodstuffs A23; obtaining protein compositions for foodstuffs, working-up proteins for foodstuffs A23J; preparations for medicinal purposes A61K; peptides containing beta-lactam rings C07D; cyclic dipeptides not having in their molecule any other peptide link than those which form their ring, e.g. piperazine-2,5-diones, C07D; ergot alkaloids of the cyclic peptide type C07D519/02; macromolecular compounds having statistically distributed amino acid units in their molecules, i.e. when the preparation does not provide for a specific; but for a random sequence of the amino acid units, homopolyamides and block copolyamides derived from amino acids C08G69/00; macromolecular products derived from proteins C08H1/00; preparation of glue or gelatine C09H; single cell proteins, enzymes C12N; genetic engineering processes for obtaining peptides C12N15/00; compositions for measuring or testing processes involving enzymes C12Q; investigation or analysis of biological material G01N33/00)
- G01N: INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES (measuring or testing apparatus or processes other than immunoassay, involving enzymes or microorganisms C12M, C12Q)
- A61K: PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES (devices or methods specially adapted for bringing pharmaceutical products into particular physical or administering forms A61J3/00; chemical aspects of, or use of materials for deodorisation of air, for disinfection or sterilisation, or for bandages, dressings, absorbent pads or surgical articles A61L; soap compositions C11D)
- Y10S: TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS

## Abstract

A method and apparatus for washing the solid annular pieces used in immune methods, i.e. the so-called EIA-rings, and for coating such rings with a reagent, as well as for the dispensing of the rings. The EIA-rings are fitted, one after the other, into a tubular container part, which is open at both ends. To the bottom end of the container part an intake tip is attached, and to the other end a device for drawing and exhausting a liquid through the intake tip into the container part. The container part enclosing the EIA-rings is then sealed at both ends until the upper end of the container part is opened for dispensing the rings. The opened end is connected to a dosage device, the intake tip of the container part is removed and the EIA-rings are dispensed by means of the device.

## Claims

### Claim 1

A method of contacting with a liquid solution annular pieces used in research and for storing and dispensing same, comprising the steps of:
(I) the liquid contacting steps including:
(a) fitting said annular pieces in a tubular container means, said container means stacking said annular pieces, said container means having upper and lower ends and being open at both ends and including retention means at the lower end for preventing said annular pieces from falling from said container;
(b) fitting said upper end of said container to a liquid intake and discharge means;
(c) fitting said lower end of said container means with releasable tip means;
(d) drawing said liquid solution into said container means by said liquid intake and discharge means;
(e) exhausting said liquid solution from said container by said liquid intake and discharge means;
(II) the storing steps including:
(f) removing said liquid intake and discharge means from said container means;
(g) releasably capping the upper end of said container means and the lower end of said tip means;
(III) the dispensing steps including:
(h) removing the caps and tip means;
(i) fitting said container means to a dispensing means, said dispensing means including means for selectively overcoming said retention means; and
(j) dispensing said rings from said container.

### Claim 2

A device for storing, contacting with a liquid and dispensing annular pieces, said device comprising:
(a) tubular container means for holding a stack of annular pieces, said tubular means having upper and lower portions, said lower portion of said tubular means including retention means to prevent said stack from falling from said container;
(b) intake tip means releasably engagable with the lower portion of said container means, said tip means including an orifice for liquid passage;
(c) syringe means for drawing and exhausting liquid into and out of said container means to contact said pieces with said liquid, said syringe means being releasably engagable with said container means;
(d) dispensing means for displacing said rings downwardly out of said container means and overcoming said retention means to dispense said rings, said dispensing means being releasably engagable with said container means; and
(e) cap means releasably engagable with the upper end of said container means and the lower end of said tip means to permit said container to be stored.

### Claim 3 (depends on 2)

The device as claimed in claim 2, wherein said dispensing means includes a handle portion, rod means slidably displaceable within said handle portion and extending out of said handle portion, the external portion of said rod means insertable within said container means and engagable with said stack of annular pieces, and manually operable means for sliding said rod means to displace and dispense said annular pieces.