# US 4,397,953 A · Method and apparatus for anaerobic fermentation > Agents: this data is available as JSON from the patentgrep API and MCP server — see https://patentgrep.com/llms.txt - Number: US 4,397,953 A - Document ID: US4397953A - Status: Granted - Kind: A - Application number: 06/235,134 - Filed: 1981-02-17 - Granted: 1983-08-09 - Earliest claimed: 1980-02-26 - Assignees: Process Engineering Company S A - Inventors: Bruno Guazzone; Felix Muller - Web page: https://patentgrep.com/patent/US4397953A ## CPC - C12M 21/04: {for producing gas, e.g. biogas (digesters for manure with production of biogas A01C3/028, biological treatment of water, waste water or sewage C02F3/00, C02F11/02, preparation of natural gas or syngas C10L3/06, C10L3/10)} - C12M 27/22: {Perforated plates, discs or walls} - C12M 29/18: {External loop; Means for reintroduction of fermented biomass or liquid percolate (loop type reactors for chemical or physical processes B01J19/2435)} - Y10S 435/801: Anerobic cultivation - C12M: APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; {APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS} - Y10S: TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS ## Abstract Anaerobic fermentation of substrates with microorganisms is carried out by a process and with an apparatus wherein fermentation is conducted in a container having an upper cylindrical section, a lower conical section, and between the upper and lower sections an apertured bottom wall containing a plurality of openings. During fermentation, the substrate and microorganisms are recirculated from an outlet in the upper cylindrical section to an inlet in the lower conical section and upward in the container through the openings in the apertured bottom wall at sufficient fluid velocity to agitate the substrate and microorganisms therein and prevent settling of the microorganisms in the container. The apertured bottom wall serves as the sole means of agitation, and the use of this process and apparatus enables a significant reduction in the usual fermentation period. ## Claims ### Claim 1 A method of submerged anerobic fermentation of substrates with microorganisms, comprising the steps of introducing microorganisms and a substrate for the microorganisms into a lower conical section and an apertured bottom wall containing a plurality of openings arranged between the lower conical section and the upper cylindrical section; causing the microorganisms to undergo submerged anaerobic fermentation in the substrate; preventing settling of microorganisms in the container during fermentation by recirculating the substrate and microorganisms by withdrawing the fermenting substrate with microorganisms therein from an outlet opening in the upper cylindrical section, and supplying the withdrawn substrate with microorganisms therein into the lower conical section through an inlet opening below said bottom wall at sufficient velocity and quantity to drive the substrate and microorganisms upward in the fermentation container and up through the openings in the apertured bottom wall at sufficient fluid velocity to prevent settling of the microorganisms in the fermentation container, and said substrate and microorganisms being agitated solely by said recirculating through said openings in the apertured bottom wall. ### Claim 2 (depends on 1) Method according to claim 1, wherein the withdrawing step includes withdrawing the substrate from different levels in the upper cylindrical container section. ### Claim 3 (depends on 1) Method according to claim 1, wherein the openings in the apertured bottom wall one arranged so that the direction of flow above the apertured bottom wall at the periphery of the container is opposite the direction of flow in the center of the container above the apertured bottom wall. ### Claim 4 (depends on 1) Method according to claim 1, wherein the anaerobic microorganisms produce a gas during the fermentation. ### Claim 5 A method of submerged anaerobic fermentation of substrates with microorganisms, comprising the steps of introducing microorganisms and a substrate for the microorganisms into a fermentation container having an upper cylindrical section, a lower conical section and an apertured bottom wall containing a plurality of openings arranged between the lower conical section and the upper cylindrical section; causing the microorganisms to undergo submerged anaerobic fermentation in the substrate; preventing settling of microorganisms in the container during fermentation by recirculating the substrate and microorganisms by withdrawing the fermenting substrate with microorganisms therein from an outlet opening in the upper cylindrical section, and supplying the withdrawn substrate with microorganisms therein into the lower conical section through an inlet opening below said bottom wall at sufficient velocity and quantity to drive the substrate and microorganisms upward in the fermentation container and up through the openings in the apertured bottom wall at sufficient fluid velocity to prevent settling of the microorganisms in the fermentation container, said plurality of openings in the bottom wall having a total opening area equal to the cross-sectional area of said inlet opening in the lower conical section and said substrate and microorganisms being agitated solely by said recirculating through said openings in the apertured bottom wall. ### Claim 6 An apparatus for submerged anaerobic fermentation of substrates with microorganisms, consisting essentially of a fermentation container for containing a substrate and microorganisms having an upper cylindrical section, a lower conical section, an apertured bottom wall containing a plurality of openings arranged between the lower conical section and the upper cylindrical section, a means for withdrawing substrate and microorganisms from the upper cylindrical section through an outlet opening therein, and means for supplying the withdrawn substrate and microorganisms at sufficient velocity and quantity into the lower conical section through an inlet opening therein below the bottom wall to drive the substrate and microorganisms upward in the container and up through the openings in the apertured bottom wall at sufficient fluid velocity to prevent settling of the microorganisms in the fermentation container, said apertured bottom wall being the sole means contained by said apparatus for agitation of substrate and microorganisms in said fermentation container. ### Claim 7 (depends on 6) Apparatus according to claim 6, wherein the supplying means includes one or more tubes connected to the inlet opening of the lower conical section. ### Claim 8 (depends on 6) Apparatus according to claim 6, wherein the openings of the apertured bottom wall are cone-shaped. ### Claim 9 (depends on 6) Apparatus according to claim 6, wherein the openings of the apertured bottom wall are circular- or rectangular-shaped. ### Claim 10 (depends on 6) Apparatus according to claim 6, wherein the openings of the apertured bottom wall are formed into curved tubes or elbows. ### Claim 11 (depends on 7) Apparatus according to claim 7, wherein the sum of all the hole areas of the openings of the apertured bottom wall is equal to the size of the cross-sectional area of the tubes connected to the inlet opening of the lower conical section. ### Claim 12 An apparatus for submerged anaerobic fermentation of substrates with microorganisms, consisting essentially of a fermentation container for containing a substrate and microorganisms having an upper cylindrical section, a lower conical section, an apertured bottom wall containing a plurality of openings arranged between the lower conical section and the upper cylindrical section, a means for withdrawing substrate and microorganisms from the upper cylindrical section through an outlet opening therein, and means for supplying the withdrawn substrate and microorganisms at sufficient velocity and quantity into the lower conical section through an inlet opening therein below the bottom wall to drive the substrate and microorganisms upward in the container and up through the openings in the apertured bottom wall at sufficient fluid velocity to prevent settling of the microorganisms in the fermentation container, said plurality of openings in the apertured bottom wall having a total opening area equal to the cross-sectional area of said inlet opening in the lower conical section.