# US 4,397,952 A · Process for obtaining glucose dehydrogenase and micro-organism therefor

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- Number: US 4,397,952 A
- Document ID: US4397952A
- Status: Granted
- Kind: A
- Application number: 06/260,480
- Filed: 1981-05-04
- Granted: 1983-08-09
- Earliest claimed: 1980-05-21
- Assignees: Boehringer Mannheim GmbH
- Inventors: Detlef von Hoerschelmann; Hans Seidel; Gerhard Berger; Armin Masuth; Klaus Beaucamp; Wolfgang Gruber
- Web page: https://patentgrep.com/patent/US4397952A

## CPC

- C12N 9/0006: {acting on CH-OH groups as donors (1.1)}
- C12N 1/205: Bacterial isolates
- C12Q 1/54: involving glucose or galactose
- C12R 2001/085: Bacillus cereus
- Y10S 435/834: Bacillus cereus
- C12N: MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA (microbiological testing media C12Q1/00)
- C12Q: MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS (immunoassay G01N33/53); COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12R: INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
- Y10S: TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS

## Abstract

The present invention provides a process for obtaining glucose dehydrogenase by culturing a micro-organism in an appropriate nutrient medium and obtaining the enzyme from the cell mass or from the culture medium, wherein the micro-organism cultured is Bacillus cereus DSM 1644. The nutrient medium used contains a carbon source which consists predominantly or solely of glycerol.

## Claims

### Claim 1

Process for obtaining glucose dehydrogenase which comprises culturing the micro-organism Bacillus cereus DSM 1644 in a nutrient medium and obtaining glucose dehydrogenase from the cell mass or from the culture medium.

### Claim 2 (depends on 1)

Process as claimed in claim 1 wherein the carbon source of the nutrient medium consists predominantly or solely of glycerol.

### Claim 3 (depends on 2)

Process as claimed in claim 2 wherein the nutrient medium contains, per liter:
5 to 50 g. glycerol,
1 to 10 g. yeast extract,
1 to 50 g. peptone,
1 to 20 g. ammonium sulphate,
0.2 to 5 g. diptassium phosphate,
0.1 to 5 g. magnesium sulphate,
1 to 10 g. sodium chloride,
as well as trace elements.

### Claim 4

A biologically pure culture of Bacillus cereus DSM 1644.