# US 4,397,950 A · Process for production of antibiotic U-64,767 using Streptomyces macronensis NRRL 12566

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- Number: US 4,397,950 A
- Document ID: US4397950A
- Status: Granted
- Kind: A
- Application number: 06/324,246
- Filed: 1981-11-23
- Granted: 1983-08-09
- Earliest claimed: 1981-11-23
- Assignees: The Upjohn Company
- Inventors: Lester A. Dolak; Fritz Reusser; Thomas M. Castle; Betty R. Hannon; Alice L. Laborde; Charles K. Marschke
- Web page: https://patentgrep.com/patent/US4397950A

## CPC

- C12P 1/06: by using actinomycetales
- C12N 1/205: Bacterial isolates
- C12R 2001/465: Streptomyces
- Y10S 435/886: Streptomyces
- C12P: FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE {(brewing of beer C12C; producing vinegar C12J; producing specific peptides or proteins C07K; producing enzymes C12N9/00; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification C12N15/00; measuring or testing processes involving enzymes or microorganisms C12Q; measuring or testing processes involving nucleic acid amplification reactions C12Q1/6844; fermentation processes to form a food composition, A21 or A23; compounds in general, see the relevant compound class, e.g. C01, C07)}
- C12N: MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA (microbiological testing media C12Q1/00)
- C12R: INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
- Y10S: TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS

## Abstract

Novel antibiotic U-64,767 producible in a fermentation under controlled conditions using a biologically pure culture of the microorganism Streptomyces macronensis Dietz sp.n., NRRL 12566. This antibiotic is active against various Gram-positive bacteria, for example, Staphylococcus aureus and Streptococcus pyogenes. It is also active against the Gram-negative bacterium Streptococcus pneumoniae. Thus, antibiotic U-64,767 can be used in various environments to eradicate or control such bacteria.

## Claims

### Claim 1

A process for preparing antibiotic U-64,767 which in its essentially pure crystalline form has the following characteristics:
(a) molecular weight of 1191 (mass spectrometry);
(b) color and form of pure solid: white
(c) is highly soluble in 1:1 water:methanol and in glacial acetic acid and poorly soluble in methanol or water alone;
(d) a characteristic .sup.13 C-NMR spectrum as shown in FIG. 3 of the drawings;
(e) a characteristic UV spectrum with a maximum absorption at 223 nm
(f) a characteristic infrared absorption spectrum when dissolved in a mineral oil mull as shown in FIG. 1 of the drawings;
(g) has a molecular formula C.sub.57 H.sub.109 N.sub.1 O.sub.24 ; and
(h) a melting point of 153.degree.-155.degree. C. with decomposition, which comprises cultivating Streptomyces macronensis Dietz sp.n., NRRL 12566, in an aqueous nutrient medium under aerobic conditions until substantial antibiotic U-64,767 activity is imparted to said medium.

### Claim 2 (depends on 1)

A process, according to claim 1, wherein said aqueous nutrient medium contains a source of assimilable carbohydrate and assimilable nitrogen.

### Claim 3 (depends on 2)

A process for recovering antibiotic U-64,767 from a fermentation beer according to claim 2 which comprises:
(a) filtering said beer to obtain filtered beer containing antibiotic U-64,767;
(b) adsorbing antibiotic U-64,767 from said filtered beer by passing said beer through a non-ionic macroporous resin;
(e) eluting antibiotic U-64,767 from said resin with a solvent for antibiotic U-64,767 to obtain an eluate containing antibiotic U-64,767; and
(d) purifying said elutate by chromatographic means to obtain essentially pure antibiotic U-64,767.

### Claim 4

A biologically pure culture of the microorganism Streptomyces macronensus Dietz sp.n., NRRL 12566.