# US 4,396,714 A · N-Sulfoalkylaniline derivatives

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- Number: US 4,396,714 A
- Document ID: US4396714A
- Status: Granted
- Kind: A
- Application number: 06/278,321
- Filed: 1981-06-29
- Granted: 1983-08-02
- Earliest claimed: 1980-01-09
- Assignees: Dojindo Laboratories
- Inventors: Masanobu Maeda; Yuko Murao
- Web page: https://patentgrep.com/patent/US4396714A

## CPC

- C12Q 1/28: involving peroxidase
- G01N 33/52: Use of compounds or compositions for colorimetric, spectrophotometric or fluorometric investigation, e.g. use of reagent paper {and including single- and multilayer analytical elements (immunological elements G01N33/54386; involving labelled immunochemicals G01N33/58; for haemoglobin or occult blood G01N33/72)}
- C12Q 2326/00: Chromogens for determinations of oxidoreductase enzymes
- Y10T 436/144444: Glucose
- Y10T 436/148888: Uric acid
- Y10T 436/206664: Ozone or peroxide
- C12Q: MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS (immunoassay G01N33/53); COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- G01N: INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES (measuring or testing apparatus or processes other than immunoassay, involving enzymes or microorganisms C12M, C12Q)
- Y10T: TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION

## Abstract

N-sulfoalkylaniline derivatives in which at least one sulfoalkyl of 2 to 4 carbon atoms is attached to the nitrogen atom which are useful as dyestuff forming substances, a composition for determining the presence of peroxides using such compounds and the process for determination of peroxides using such compounds.

## Claims

### Claim 1

A composition for determing the presence of peroxides, comprising a crystalline compound of the formula (I): ##STR5## wherein R.sup.1 is selected from the group consisting of hydrogen, lower alkyl and sulfoalkyl of 2-4 carbon atoms;
R.sup.2 is sulfoalkyl of 2-4 carbon atoms;
R.sup.3 is selected from the group consisting of hydrogen, hydroxy and lower alkyl and lower alkoxy; and
R.sup.4 is selected from the group consisting of hydrogen, lower alkoxy and lower alkyl, or the alkali metal or alkaline earth metal salt thereof, and a color-developer substance that will react with hydrogen peroxide selected from the groups consisting of 4-aminoantipyrine and 3-methyl-benzothiazolinonehydrazone and a said compound to form a dyestuff in the presence of peroxidase or a transition metal.

### Claim 2 (depends on 1)

A composition according to claim 1 wherein the compound of the formula (I) is the calcium salt of N-ethyl-N-sulfopropyl-3-methylaniline.

### Claim 3 (depends on 1)

A composition according to claim 1 wherein the compound of the formula (I) is the calcium salt of N-ethyl-N-sulfopropylaniline.

### Claim 4 (depends on 1)

A composition according to claim 1 wherein the compound of the formula (I) is the sodium salt of N-sulfopropyl-2-methoxyaniline.

### Claim 5

A process for determination of peroxides, which comprises adding to a sample containing an unknown quantity of a material capable of liberating peroxide upon peroxidation, a crystalline compound of the formula (I): ##STR6## wherein R.sup.1 is selected from the group consisting of hydrogen, lower alkyl and sulfoalkyl of 2-4 carbon atoms;
R.sup.2 is sulfoalkyl of 2-4 carbon atoms;
R.sup.3 is selected from the group consisting of hydrogen, hydroxy and lower alkyl and lower alkoxy; and
R.sup.4 is selected from the group consisting of hydrogen, lower alkoxy and lower alkyl, or the alkali metal or alkaline earth metal salt thereof, a color-developer substance that will react in an oxidative-condensation with said compound to form a dyestuff and a peroxidase or a transition metal, and measuring the absorbency of the solution at maximum absorption.

### Claim 6 (depends on 5)

The process according to claim 5, wherein said color developer is 4-aminoantipyrine or 3-methylbenzothiazolinonehydrazone.

### Claim 7 (depends on 5)

A process according to claim 5 wherein the compound of the formula (I) is the calcium salt of N-ethyl-N-sulfopropyl-3-methylaniline.

### Claim 8 (depends on 5)

A process according to claim 5 wherein the compound of the formula (I) is the calcium salt of N-ethyl-N-sulfopropylaniline.

### Claim 9 (depends on 5)

A process according to claim 5 wherein the compound of the formula (I) is the sodium salt of N-sulfopropyl-2-methoxyaniline.