Fluorescent composition, a process for synthesizing the fluorescent composition
AssigneeVarian Associates, Inc.
InventorSeth R. Abbott
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Abstract
A fluorescent composition useful in thin-layer chromatography comprises an inert porous particle having a fluorescent material fixedly attached by covalent chemical bonding to the surface thereof. A process for synthesizing this fluorescent composition is disclosed. A method for detecting the radioactivity of a radioactive species using a cell packed with such a fluorescent composition is also disclosed.
Claims27
A process for the manufacture of a fluorescent composition comprising an inert porous particle having a size of about 5 to 20 microns, and a fluorescent material;
- said fluorescent material being fixedly attached by covalent chemical bonding to the surface of said particles;
- wherein said particle is microparticulate silica;
- said process comprising steps of:
- (a) reacting at room temperature an amine linking agent with an aqueous slurry of said silica whereby there is formed an intermediate product consisting of said amine linking agent covalently bonded to said silica;
- (b) recovering, washing and drying said intermediate product;
- (c) reacting at room temperature a slurry of the dried intermediate product with said fluorescent material whereby said fluorescent composition is produced;
- and
- (d) recovering, washing and drying said fluorescent composition.
A fluorescent composition useful for detection of radioactive or non-fluorescent species by fluorescence, said composition comprising microparticulate silica having a size of about 5 to 20 microns, an amine linking agent fixedly attached by covalent chemical bonding to the surface of said microparticulate silica by reacting said microparticulate silica with said amine linking agent to form an intermediate product, and a fluorescent material fixedly attached to said amine linking agent by reacting said fluorescent material with the said intermediate product, said microparticulate silica-amine linking agent reaction comprising (a) reacting at room temperature an amine linking agent with an aqueous slurry of said silica whereby there is formed an intermediate product consisting of said amine linking agent covalently bonded to said silica and (b) recovering, washing and drying said intermediate product, and said fluorescent materialintermediate product reaction comprising (c) reacting at about room temperature a slurry of the dried intermediate product with said fluorescent material whereby said fluorescent composition is produced, and (d) recovering, washing and drying said fluorescent composition.
Dependencies are inferred from the claim text.