# US 4,395,490 A · Process for production of improved maltase

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- Number: US 4,395,490 A
- Document ID: US4395490A
- Status: Granted
- Kind: A
- Application number: 06/329,449
- Filed: 1981-12-10
- Granted: 1983-07-26
- Earliest claimed: 1980-08-13
- Assignees: Massachusetts Institute of Technology
- Inventors: Charles L. Cooney; Eugene J. Schaefer
- Web page: https://patentgrep.com/patent/US4395490A

## CPC

- C12N 9/2408: {acting on alpha -1,4-glucosidic bonds}
- C12N 1/185: Saccharomyces isolates
- C12R 2001/85: Saccharomyces
- Y10S 435/94: Saccharomyces
- C12N: MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA (microbiological testing media C12Q1/00)
- C12R: INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
- Y10S: TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS

## Abstract

Maltase is produced by growing a mutant of the yeast strain Saccharomyces italicus capable of growing in a growth medium utilizing sucrose as the carbon source. Maltase production is optimized by controlling time of fermentation, growth medium composition and sonication time during maltase recovery.

## Claims

### Claim 1

In a process for forming maltase utilizing Saccharomyces italicus in a growth medium, said Saccharomyces italicus being obtained by mutation of Saccharomyces italicus and having the property of producing maltase while grown on a carbon source selected from the group consisting of maltose, glycerol, acetate, fructose, glucose and sucrose, the improvement which comprises utilizing in the growth medium yeast extract at a concentration of between about 1 and 15 g/L.

### Claim 2 (depends on 1)

The process of claim 1 wherein the concentration of yeast extract is between about 2 and 5 g/L.

### Claim 3 (depends on 1)

The process of claim 1 wherein the maltase is recovered by sonication of cells of said yeast between about 1 and 6 minutes.

### Claim 4 (depends on 1)

The process of claim 1 wherein the sonication time is between about 2 and 4 minutes.

### Claim 5 (depends on 1)

The process of claim 1 wherein said growth medium contains sucrose at a concentration between about 5 and 50 g/L.

### Claim 6 (depends on 1)

The process of claim 1 wherein the said growth medium contains sucrose at a concentration between about 40 and 45 g/L.

### Claim 7 (depends on 1, 2, 3, 4, 5, 6)

The process of any one of claims 1, 2, 3, 4, 5 or 6 wherein the growth medium is continuously added to said Saccharomyces italicus in a growth vessel and removing Saccharomyces italicus containing maltase for said culture vessel.

### Claim 8 (depends on 1, 2, 3, 4, 5, 6)

The process of any one of claims 1, 2, 3, 4, 5 or 6 wherein said Saccharomyces italicus is Saccharomyces italicus ATCC 20601.