# US 4,395,489 A · Process for the recovery of glycerol dehydrogenase

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- Number: US 4,395,489 A
- Document ID: US4395489A
- Status: Granted
- Kind: A
- Application number: 06/218,138
- Filed: 1980-12-19
- Granted: 1983-07-26
- Earliest claimed: 1979-12-27
- Assignees: Boehringer Mannheim GmbH
- Inventors: Peter Stahl; Hans Seidel; Herwig Brunner
- Web page: https://patentgrep.com/patent/US4395489A

## CPC

- C12N 9/0006: {acting on CH-OH groups as donors (1.1)}
- Y10S 435/828: Aerobacter
- C12N: MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA (microbiological testing media C12Q1/00)
- Y10S: TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS

## Abstract

Process for obtaining high yields of glycerol dehydrogenase from micro-organisms and glycerol dehydrogenase having a low K.sub.M value.

## Claims

### Claim 1

Process for obtaining glycerol dehydrogenase from micro-organisms, which process comprises culturing a glycerol dehydrogenase-forming Aerobacter aerogenes micro-organism in a glycerol-containing nutrient medium, culturing the micro-organism first under aerobic conditions, up to the end of the log phase, thereafter under anaerobic conditions, during which more glycerol is added to the nutrient medium than is used up by the micro-organism, and then isolating the glycerol dehydrogenase formed from the biomass or from the nutrient medium.

### Claim 2 (depends on 1)

Process as claimed in claim 1 wherein the micro-organism is Aerobacter aerogenes DSM 1643.

### Claim 3 (depends on 1)

Process as claimed in claim 1 wherein the micro-organism is Aerobacter aerogenes NCIB 418.

### Claim 4 (depends on 1)

Process as claimed in claim 1 wherein the glycerol content of the nutrient medium in the anaerobic phase is increased from 0.3 to 0.5 up to 0.8 to 1.5%.

### Claim 5 (depends on 1)

Process as claimed in claim 1 wherein the biotin is added to the nutrient medium.

### Claim 6 (depends on 1)

Process as claimed in claim 1 wherein culturing is carried out at a temperature of from 25.degree. to 40.degree. C.

### Claim 7

Process for obtaining glycerol dehydrogenase from micro-organisms, which process comprises culturing an Aerobacter aerogenes DSM 1643 in an appropriate glycerol-containing nutrient medium, and then isolating the glycerol dehydrogenase formed from the biomoass or from the nutrient medium.

### Claim 8

Glycerol dehydrogenase with a K.sub.M value of 6.6.times.10.sup.-4 M.

### Claim 9

Process for obtaining glycerol dehydrogenase from micro-organisms, which process comprises culturing a glycerol dehydrogenase-forming micro-organism selected from the group consisting of Aerobacter aerogenes DSM 1643 and NCIB 418 in a glycerol-containing nutrient medium, culturing the micro-organism first under aerobic conditions up to the end of the log phase and thereafter under anaerobic conditions and then isolating the glycerol dehydrogenase formed from the biomass or from the nutrient medium.

### Claim 10 (depends on 9)

Process as claimed in claim 9 wherein more glycerol is added to the nutrient medium in the anaerobic culture phase than is used up by the micro-organism.

### Claim 11 (depends on 9)

Process as claimed in claim 9 wherein the glycerol content of the nutrient medium in the anaerobic phase is increased from 0.3 to 0.5 up to 0.8 to 1.5%.

### Claim 12 (depends on 9)

Process as claimed in claim 9 wherein biotin is added to the nutrient medium.

### Claim 13 (depends on 9)

Process as claimed in claim 9 wherein culturing is carried out at a temperature of from 25.degree. to 40.degree. C.

### Claim 14 (depends on 7)

Glycerol dehydrogenase according to the process of claim 7 and having a K.sub.M value of 6.6.times.10.sup.-4 M.