# US 4,394,487 A · Adsorbent for the affinity-specific separation of macromolecular materials

> Agents: this data is available as JSON from the patentgrep API and MCP server — see https://patentgrep.com/llms.txt

- Number: US 4,394,487 A
- Document ID: US4394487A
- Status: Granted
- Kind: A
- Application number: 06/309,543
- Filed: 1981-10-07
- Granted: 1983-07-19
- Earliest claimed: 1977-03-02
- Assignees: Boehringer Mannheim GmbH
- Inventors: Werner Muller; Hans Bunemann
- Web page: https://patentgrep.com/patent/US4394487A

## CPC

- B01J 20/3255: {comprising a cyclic structure containing at least one of the heteroatoms nitrogen, oxygen or sulfur, e.g. heterocyclic or heteroaromatic structures}
- B01D 15/3804: {Affinity chromatography}
- B01J 20/3219: {involving a particular spacer or linking group, e.g. for attaching an active group}
- B01J 20/3253: {comprising a cyclic structure not containing any of the heteroatoms nitrogen, oxygen or sulfur, e.g. aromatic structures}
- C07D 219/12: Amino-alkylamino radicals attached in position 9
- C07D 221/12: Phenanthridines
- C08F 265/10: on to polymers of amides or imides
- C12N 15/1006: {by means of a solid support carrier, e.g. particles, polymers}
- C12N 15/101: {by chromatography, e.g. electrophoresis, ion-exchange, reverse phase}
- B01J: CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
- B01D: SEPARATION (separating solids from solids by wet methods B03B, B03D; by pneumatic jigs or tables B03B; by other dry methods B07; magnetic or electrostatic separation of solid materials from solid materials or fluids, separation by high-voltage electric fields B03C; centrifuges, vortex apparatus B04; presses per se for squeezing-out liquid from liquid-containing material B30B9/02; treatment of water C02F, e.g. softening by ion-exchange C02F1/42; {arrangements of air intake cleaners in gas turbine plants F02C7/05} ; arrangements or mounting of filters in air-conditioning, air-humidification or ventilation F24F13/28)
- C07D: HETEROCYCLIC COMPOUNDS (macromolecular compounds C08)
- C08F: MACROMOLECULAR COMPOUNDS OBTAINED BY REACTIONS ONLY INVOLVING CARBON-TO-CARBON UNSATURATED BONDS
- C12N: MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA (microbiological testing media C12Q1/00)

## Abstract

Adsorbents are provided for the affinity-specific separation of macromolecular materials, particularly biopolymers, the adsorbents comprising a polymeric carrier on to which an affine residue for the macromolecular material is covalently bound, either directly or via polymeric spacer. Preferred affine residue is comprised of a specific complex formers which are base-specific and/or structure specific for the macromolecular material. The invention also provides novel complex formers comprising a certain dyestuff residues and processes for their preparation.

## Claims

### Claim 1

Process for the preparation of an adsorbent for the affinity-specific separation of nucleic acids, comprising a polymeric carrier material onto which an affine residue group consisting of a base and/or structure specific complex former for nucleic acids, is covalently bound wherein said polymeric carrier material is first formed from at least one monomer which additionally possesses a functional group via which the affine residue for the biopolymer can be bound directly or via a polymeric spacer, the polymeric carrier material is optionally comminuted to the desired particle size and then the affine residue for the biopolymer is grafted on either directly or by copolymerization in the presence of at least one further monomer as co-monomer so that particles of the desired size are produced.

### Claim 2 (depends on 1)

Process as claimed in claim 1 wherein the affine residue for the biopolymer having a copolymerizable double bound is grafted on to poly-bisacrylamide in the presence of an excess of a further copolymerizable monomer as co-monomer.

### Claim 3 (depends on 1)

Process as claimed in claim 1 wherein the graft copolymerization is carried out in water or in an inert solvent in the presence of a conventional polymerization catalyst.

### Claim 4 (depends on 1)

Process as claimed in claim 1 wherein the affine residue for the biopolymer having a copolymerizable double bond is acrylic-phenyl neutral red or acrylic-malachite green and the further copolymerizable monomer used is acrylamide.

### Claim 5 (depends on 1)

Process as claimed in claim 1 wherein a copolymer of acrylamide and acrylic-phenyl neutral red or acrylic-malachite green with a degree of polymerization of 200 to 300 is grafted on.

### Claim 6 (depends on 1)

Process as claimed in claim 1 wherein the affine residue for the biopolymer having a copolymerizable double bond and the further copolymerizable monomer are used in a weight ratio of 1:100 to 1:5000.

### Claim 7 (depends on 6)

Process as claimed in claim 6 wherein the said weight ratio is preferably 1:3000.