# US 4,394,447 A · Production of high-pyruvate xanthan gum on synthetic medium

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- Number: US 4,394,447 A
- Document ID: US4394447A
- Status: Granted
- Kind: A
- Application number: 05/908,601
- Filed: 1978-05-23
- Granted: 1983-07-19
- Earliest claimed: 1978-05-23
- Assignees: The United States of America as represented by the Secretary of Agriculture
- Inventors: Martin C. Cadmus; Clarence A. Knutson, Jr.
- Web page: https://patentgrep.com/patent/US4394447A

## CPC

- C12P 19/06: Xanthan, i.e. Xanthomonas-type heteropolysaccharides
- Y10S 435/91: Xanthomonas
- C12P: FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE {(brewing of beer C12C; producing vinegar C12J; producing specific peptides or proteins C07K; producing enzymes C12N9/00; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification C12N15/00; measuring or testing processes involving enzymes or microorganisms C12Q; measuring or testing processes involving nucleic acid amplification reactions C12Q1/6844; fermentation processes to form a food composition, A21 or A23; compounds in general, see the relevant compound class, e.g. C01, C07)}
- Y10S: TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS

## Abstract

A high-pyruvate xanthan gum, substantially free of insolubles and undesirable coloration, is produced by fermentation of Xanthomonas campestris on a carbohydrate-containing nutrient medium having (NH.sub.4).sub.2 HPO.sub.4 at a level of at least 0.15% as the primary nitrogen source, and having a total phosphate level of at least about 0.25%.

## Claims

### Claim 1

In a process for producing polysaccharides by fermentation of a carbohydrate-containing nutrient medium with the bacterium Xanthomonas campestris, wherein said bacterium is cultivated on said medium under conditions suitable for its growth, the improvement comprising:
a. providing (NH.sub.4).sub.2 HPO.sub.4 as the primary nitrogen source in said nutritent medium at a level of at least 0.15 g./100 ml. of medium;
b. providing a total phosphate level in said nutrient medium of at least 0.25 g./100 ml. of medium; and
c. recovering from said fermentation medium a polysaccharide isolated from residual solids in said medium wherein said polysaccharide has a pyruvic acid content of at least 3.3% by weight.

### Claim 2 (depends on 1)

The process as defined in claim 1 wherein said (NH.sub.4).sub.2 HPO.sub.4 is provided in an amount of 0.15-0.25 g./100 ml. of medium.

### Claim 3 (depends on 1)

The process as defined in claim 1 wherein said nutrient medium comprises dipotassium phosphate or disodium phosphate.