# US 4,394,377 A · Ruminant animal performance by co-administering choline and propionate enchancers > Agents: this data is available as JSON from the patentgrep API and MCP server — see https://patentgrep.com/llms.txt - Number: US 4,394,377 A - Document ID: US4394377A - Status: Granted - Kind: A - Application number: 06/289,001 - Filed: 1981-07-31 - Granted: 1983-07-19 - Earliest claimed: 1981-07-31 - Assignees: Syntex (U.S.A.) Inc. - Inventors: Howard R. Spires - Web page: https://patentgrep.com/patent/US4394377A ## CPC - A61K 31/35: having six-membered rings with one oxygen as the only ring hetero atom - A61K 38/14: Peptides containing saccharide radicals; Derivatives thereof {, e.g. bleomycin, phleomycin, muramylpeptides or vancomycin} - A61K: PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES (devices or methods specially adapted for bringing pharmaceutical products into particular physical or administering forms A61J3/00; chemical aspects of, or use of materials for deodorisation of air, for disinfection or sterilisation, or for bandages, dressings, absorbent pads or surgical articles A61L; soap compositions C11D) ## Abstract The availability of supplemental dietary choline to animals with a developed rumen is increased by co-administering choline or choline compounds with a propionate-enhancing antibiotic compound. ## Claims ### Claim 1 A method for increasing the availability of supplemental dietary choline to an animal with a developed rumen, which method comprises co-administering a diet-supplementing amount of choline or a choline compound with a propionate-enhancing amount of a propionate-enhancing antibiotic compound. ### Claim 2 (depends on 1) The method of claim 1 wherein said compound is a polyether ionophore or a peptide antibiotic compound. ### Claim 3 (depends on 2) The method of claim 2 wherein said animal is a bovine or a sheep. ### Claim 4 (depends on 3) The method of claim 3 wherein said compound is a polyether ionophore co-administered in an amount of 0.02-10 mg/kg/day with an amount of choline or choline compound sufficient to provide 10 to 250 mg of choline per kilogram of body weight per day. ### Claim 5 (depends on 4) The method of claim 4 wherein said compound is monensin, lasalocid, salinomycin, dianemycin, nigericin, septamycin, narasin, laidlomycin or a laidlomycin ester. ### Claim 6 (depends on 5) The method of claim 5 wherein 0.05-2.5 mg/kg/day of monensin is co-administered with an amount of choline or a choline compound sufficient to provide 20-100 mg/kg/day of choline. ### Claim 7 (depends on 5) The method of claim 5 wherein 0.05-1.5 mg/kg/day of narasin is co-administered with an amount of choline or a choline compound sufficient to provide 20-100 mg/kg/day of choline. ### Claim 8 (depends on 5) The method of claim 5 wherein 0.1 to 1.0 mg/kg/body weight of a laidlomycin ester is co-administered with an amount of choline or choline compound sufficient to provide 20-100 mg/kg/day of choline. ### Claim 9 (depends on 8) The method of claim 8 wherein 0.1 to 1.0 mg/kg/body weight of laidlomycin butyrate is co-administered with an amount of choline or choline compound sufficient to provide 20-100 mg/kg/day of choline. ### Claim 10 (depends on 3) The method of claim 3 wherein said compound is a peptide antibiotic compound co-administered in an amount of 0.05-10 mg/kg/day with an amount of choline or choline compound sufficient to provide 10-250 mg/kg/day of choline. ### Claim 11 (depends on 10) The method of claim 10 wherein said compound is AV290 or AV290-alkyl sulfate co-administered in an amount of 0.1-5.0 mg/kg/day with an amount of choline or choline compound sufficient to provide 20-100 mg/kg/day of choline. ### Claim 12 (depends on 8) The method of claim 8 wherein 0.1 to 1.0 mg/kg/body weight of laidlomycin propionate is co-administered with an amount of choline or choline compound sufficient to provide 20-100 mg/kg/day of choline. ### Claim 13 A composition for increasing the availability of supplemental dietary choline to an animal with a developed rumen, which composition comprises a diet-supplementing amount of choline or a choline compound, a propionate-enchancing amount of a propionate-enhancing antibiotic compound and a suitable carrier. ### Claim 14 (depends on 13) The composition of claim 13 which comprises choline or choline compound sufficient to provide 1-1250 parts by weight of choline for every part by weight of propionate-enhancing compound and wherein the active ingredients are present in an amount of 1 to 75 percent and the balance is an edible organic or inorganic carrier. ### Claim 15 (depends on 14) The composition of claim 14 wherein said compound is a polyether ionophore. ### Claim 16 (depends on 15) The composition of claim 15 comprising choline or choline compound sufficient to provide 1 to 250 parts choline for every part polyether ionophore. ### Claim 17 (depends on 16) The composition of claim 16 wherein said compound is a polyether ionophore which is monensin, lasalocid, salinomycin, dianemycin, nigericin, septamycin, narasin, laidlomycin or a laidlomycin ester. ### Claim 18 (depends on 17) The composition of claim 17 wherein said compound is laidlomycin butyrate. ### Claim 19 (depends on 14) The composition of claim 14 wherein said compound is a peptide antibiotic compound. ### Claim 20 (depends on 19) The composition of claim 19 comprising choline or choline compound sufficient to provide 1 to 250 parts choline for every part peptide antibiotic compound. ### Claim 21 (depends on 20) The composition of claim 20 wherein said compound is AV290 or AV290-alkyl sulfate. ### Claim 22 (depends on 17) The composition of claim 17 wherein said compound is laidlomycin propionate. ### Claim 23 A feed composition for increasing the availability of supplemental dietary choline to a ruminant animal which comprises a diet-supplementing amount of choline or a choline compound, a propionate-enchancing amount of a propionate-enhancing antibiotic compound and cattle feed. ### Claim 24 (depends on 23) The composition of claim 23 wherein said antibiotic is a polyether ionophore present in an amount of 0.714-357 grams/ton of feed and an amount of choline or choline compound sufficient to provide 0.357-8.98 kilograms of choline per ton of feed, dry matter basis. ### Claim 25 (depends on 24) The composition of claim 24 wherein said antibiotic is monensin, lasalocid, salinomycin, dianemycin, nigericin, septamycin, narasin, laidlomycin or a laidlomycin ester. ### Claim 26 (depends on 25) The composition of claim 25 wherein said antibiotic is monensin in an amount of 1.79-89 grams/ton and an amount of choline or a choline compound sufficient to provide 0.714-3.75 kg of choline/ton. ### Claim 27 (depends on 25) The composition of claim 25 wherein said antibiotic is narasin in an amount of 1.79-89 g/ton and an amount of choline or a choline compound sufficient to provide 0.714-3.75 kg of choline/ton. ### Claim 28 (depends on 25) The composition of claim 25 wherein said antibiotic is a laidlomycin ester in an amount of 1.79-89 g/ton and an amount of choline or choline compound sufficient to provide 0.714-3.75 kg of choline/ton. ### Claim 29 (depends on 25) The composition of claim 25 wherein said antibiotic is laidlomycin butyrate in an amount of 1.79-89 g/ton and an amount of choline or choline compound sufficient to provide 0.714-3.75 kg of choline/ton. ### Claim 30 (depends on 23) The composition of claim 23 wherein said compound is a peptide present in an amount of 0.714-357 g/ton and an amount of choline or choline compound sufficient to provide 0.357-8.98 kg of choline/ton. ### Claim 31 (depends on 30) The composition of claim 30 wherein said compound is AV290 or AV290-alkyl sulfate present in an amount of 1.79-89 g/ton and an amount of choline or choline compound sufficient to provide 0.714-3.75 kg of choline/ton. ### Claim 32 (depends on 25) The composition of claim 25 wherein said antibiotic is laidlomycin propionate in an amount of 1.79-89 g/ton and an amount of choline or choline compound sufficient to provide 0.714-3.75 kg of choline per ton.