# US 4,393,138 A · Method for disinfecting immobilized enzymes

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- Number: US 4,393,138 A
- Document ID: US4393138A
- Status: Granted
- Kind: A
- Application number: 06/206,099
- Filed: 1980-11-12
- Granted: 1983-07-12
- Earliest claimed: 1979-12-12
- Assignees: Corning Glass Works
- Inventors: Jean-Luc A. G. Baret
- Web page: https://patentgrep.com/patent/US4393138A

## CPC

- A01N 33/04: Nitrogen directly attached to aliphatic or cycloaliphatic carbon atoms
- A23C 21/023: {Lactose hydrolysing enzymes, e.g. lactase, B-galactosidase}
- C12P 1/00: Preparation of compounds or compositions, not provided for in groups C12P3/00 - C12P39/00, by using microorganisms or enzymes
- A01N: PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF (preservation of food or foodstuff A23); BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES (preparations for medical, dental or toiletry purposes which kill or prevent the growth or proliferation of unwanted organisms A61K); PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A23C: DAIRY PRODUCTS, e.g. MILK, BUTTER OR CHEESE; MILK OR CHEESE SUBSTITUTES; PREPARATION THEREOF (preservation thereof A23B11/00)
- C12P: FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE {(brewing of beer C12C; producing vinegar C12J; producing specific peptides or proteins C07K; producing enzymes C12N9/00; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification C12N15/00; measuring or testing processes involving enzymes or microorganisms C12Q; measuring or testing processes involving nucleic acid amplification reactions C12Q1/6844; fermentation processes to form a food composition, A21 or A23; compounds in general, see the relevant compound class, e.g. C01, C07)}

## Abstract

Disinfecting of immobilized enzymes is carried out by contacting the immobilized enzymes with a dilute aqueous solution of at least one substituted diethylenetriamine at a concentration and for a period of time which is sufficient to substantially kill the contaminating microorganisms without significant deleterious effects on the immobilized enzymes. The substituted diethylenetriamine is preferably dioctyldiethylenetriamine or a mixture of dioctyldiethylenetriamine and trioctyldiethylenetriamine.

## Claims

### Claim 1

A method for disinfecting immobilized enzymes which comprises contacting the immobilized enzymes with a dilute aqueous solution of at least one substituted diethylenetriamine at a concentration and for a period of time which is sufficient to substantially kill the contaminating microorganisms without significant deleterious effects on the immobilized enzymes, in which the enzymes are selected from the group consisting of lactase and glucose isomerase, the enzymes are immobilized on an inorganic support, and the substituted diethylenetriamine is selected from the group consisting of dioctyldiethylenetriamine and a mixture of dioctyldiethylenetriamine and trioctyldiethylenetriamine.

### Claim 2 (depends on 1)

The method of claim 1 in which the enzymes are immobilized on a particulate support.

### Claim 3 (depends on 2)

The method of claim 2 in which the immobilized enzymes are contained in a reactor having a columnar configuration and the dilute aqueous solution is passed through the column in a reverse flow mode under conditions sufficient to fluidize the particulate immobilized enzyme.

### Claim 4 (depends on 1)

The method of claim 1 in which one or more of the substituted diethylenetriamines are incorporated in the feed solution.