# US 4,393,135 A · Method for producing L-glutamic acid by fermentation

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- Number: US 4,393,135 A
- Document ID: US4393135A
- Status: Granted
- Kind: A
- Application number: 06/212,245
- Filed: 1980-12-02
- Granted: 1983-07-12
- Earliest claimed: 1979-12-10
- Assignees: Ajinomoto Co., Inc.
- Inventors: Takayasu Tsuchida; Shigeru Nakamori
- Web page: https://patentgrep.com/patent/US4393135A

## CPC

- C12P 13/14: Glutamic acid; Glutamine
- C12N 1/205: Bacterial isolates
- C12N 15/52: Genes encoding for enzymes or proenzymes
- C12R 2001/19: Escherichia coli
- Y10S 435/848: Escherichia
- Y10S 435/849: Escherichia coli
- C12P: FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE {(brewing of beer C12C; producing vinegar C12J; producing specific peptides or proteins C07K; producing enzymes C12N9/00; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification C12N15/00; measuring or testing processes involving enzymes or microorganisms C12Q; measuring or testing processes involving nucleic acid amplification reactions C12Q1/6844; fermentation processes to form a food composition, A21 or A23; compounds in general, see the relevant compound class, e.g. C01, C07)}
- C12N: MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA (microbiological testing media C12Q1/00)
- C12R: INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
- Y10S: TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS

## Abstract

An L-glutamic acid producing microorganism, which is obtained by incorporation into a host strain of the genus Escherichia of a hybrid plasmid having inserted therein a DNA fragment with genetic information controlling L-glutamic acid production, said fragment being derived from a donor strain of Escherichia which is capable of producing L-glutamic acid useful for the production of high levels of L-glutamic acid.

## Claims

### Claim 1

A method of producing L-glutamic acid by fermentation which comprises culturing in a culture medium an L-glutamic acid producing microorganism which is obtained by incorporating a hybrid plasmid having inserted therein a DNA fragment with genetic information controlling L-glutamic acid production, said fragment being derived from a donor strain of the genus Escherichia which is capable of producing L-glutamic acid, into a recipient strain of the genus Escherichia, wherein said L-glutamic acid producing microorganism is the transformant NRRL B-12286, NRRL B-12299, NRRL B-12300, NRRL B-12301, or NRRL B-12302.

### Claim 2 (depends on 1)

The method of claim 1, wherein said donor strain is a mutant resistant to a lysine-analogue.

### Claim 3 (depends on 1)

The method of claim 1, wherein said donor strain is a mutant resistant to oxa-lysine, lysine-hydroxamate, S-(2-aminoethyl)-cysteine, .gamma.-methyl-lysine, or .beta.-chloro-caprolactum.

### Claim 4 (depends on 1)

The method of claim 1, wherein said recipient is a mutant resistant to p-fluorophenylalanine, S-(2-aminoethyl)-cysteine, 2-thiazolealanine, or 1, 2, 4-triazolealanine.

### Claim 5 (depends on 1)

The method according to claim 1 wherein the L-glutamic acid producing microorganism is the transformant NRRL B-12286.

### Claim 6 (depends on 5)

The method according to claim 5 wherein the transformant is resistant to S-(2-aminoethyl)-cysteine.

### Claim 7 (depends on 1)

The method according to claim 1 wherein the L-glutamic acid producing microorganism is the transformant NRRL B-12299.

### Claim 8 (depends on 1)

The method according to claim 1 wherein the L-glutamic acid producing microorganism is the transformant NRRL B-12300.

### Claim 9 (depends on 1)

The method according to claim 1 wherein the L-glutamic acid producing microorganism is the transformant NRRL B-12301.

### Claim 10 (depends on 1)

The method according to claim 1 wherein the L-glutamic acid producing microorganism is the transformant NRRL B-12302.

### Claim 11

An L-glutamic acid producing transformant of the genus Escherichia containing a hybrid plasmid having inserted therein a DNA fragment with genetic information controlling L-glutamic acid production derived from a donor mutant of the genus Escherichia capable of producing L-glutamic acid, said mutant being resistant to lysine analog, wherein said transformant is NRRL B-12286, NRRL B-12299, NRRL B-12300, NRRL B-12301, or NRRL B-12302.

### Claim 12 (depends on 11)

The transformant according to claim 11 having the designation NRRL B-12286.

### Claim 13 (depends on 11)

The transformant according to claim 11 having the designation NRRL B-12299.

### Claim 14 (depends on 11)

The transformant according to claim 11 having the designation NRRL B-12300.

### Claim 15 (depends on 11)

The transformant according to claim 11 having the designation NRRL B-12301.

### Claim 16 (depends on 11)

The transformant according to claim 11 having the designation NRRL B-12302.