# US 4,391,910 A · Processes for producing thermophilic aspartase

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- Number: US 4,391,910 A
- Document ID: US4391910A
- Status: Granted
- Kind: A
- Application number: 06/209,494
- Filed: 1980-11-24
- Granted: 1983-07-05
- Earliest claimed: 1979-11-27
- Assignees: Kyowa Hakko Kogyo Co., Ltd.
- Inventors: Kazuo Kimura; Kenichiro Takayama; Yutaka Ado; Tamotsu Kawamoto; Izumi Masunaga
- Web page: https://patentgrep.com/patent/US4391910A

## CPC

- C12N 1/205: Bacterial isolates
- C12N 9/88: Lyases (4.)
- C12N 11/02: Enzymes or microbial cells immobilised on or in an organic carrier
- C12P 13/20: Aspartic acid; Asparagine
- C12R 2001/07: Bacillus
- Y10S 435/832: Bacillus
- C12N: MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA (microbiological testing media C12Q1/00)
- C12P: FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE {(brewing of beer C12C; producing vinegar C12J; producing specific peptides or proteins C07K; producing enzymes C12N9/00; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification C12N15/00; measuring or testing processes involving enzymes or microorganisms C12Q; measuring or testing processes involving nucleic acid amplification reactions C12Q1/6844; fermentation processes to form a food composition, A21 or A23; compounds in general, see the relevant compound class, e.g. C01, C07)}
- C12R: INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
- Y10S: TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS

## Abstract

Thermophilic aspartase is produced by culturing a microorganism belonging to the genus Bacillus. The enzyme is useful as a catalyst in the production of L-aspartic acid from ammonium fumarate or a mixture of fumaric acid and ammonia.

## Claims

### Claim 1

A process for producing thermophilic aspartase which comprises culturing a thermophilic aspartase producing Bacillus aminogenes having the identifying characterestics of NRRL B12061 or Bacillus thermoaminophilus having the identifying characteristics of NRRL B12060 in a nutrient medium until substantial enzymatic activity is detectable and thereafter isolating said thermophilic aspartase.

### Claim 2 (depends on 1)

A process according to claim 1 wherein said culturing step is carried out at a temperature of 30.degree. to 60.degree. C. and at a pH of from 6 to 7.5.

### Claim 3

A biologically pure culture of Bacillus thermoaminophilus having the identifying characteristics of NRRL B-12060, which produces thermophilic aspartase in recoverable quantities upon culturing.

### Claim 4

A biologically pure culture of Bacillus aminogenes having the identifying characteristics of NRRL B-12061, which produces thermophilic aspartase in recoverable quantities upon culturing.