Process for the production of human chorionic gonadotropin
AssigneeKabushiki Kaisha Hayashibara Seibutsu Kagaku Kenkyujo
InventorKaname Sugimoto
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Abstract
The present invention relates to a process for the production of human chorionic gonadotropin (hCG). More precisely, the invention relates to a process for the mass production of hCG, comprising in vivo multiplication of human lymphoblastoid cells capable of producing hCG, and hCG production by the multiplied human lymphoblastoid cells. The hCG production according to the invention is extremely higher, in terms of hCG production per cell, than that attained by conventional process using in vitro tissue culture; thus, hCG can be used in a sufficient amount in the prevention and treatment of human diseases.
Claims11
A process for producing human chorionic gonadotropin (hCG), which comprises:
- (1) implanting human lymphoblastoid cells capable of producing hCG into a non-human warm-blooded animal;
- feeding the animal to cause the human cells therein to multiply;
- extracting and disaggregating the resultant multiplied human cells formed in the animal;
- culturing the human cells in a nutrient medium for a period sufficient to accumulate a significant amount of hCG;
- and
- harvesting the accumulated hCG from the culture, or alternatively,
- (2) placing human lymphoblastoid cells capable of producing hCG in suspension in a diffusion chamber;
- embedding the chamber in or placing the chamber on a non-human warm-blooded animal in a manner such that the nutrient body fluid of the non-human warm-blooded animal is supplied to the cells within the chamber;
- feeding the animal to cause the human cells in the chamber to multiply;
- collecting the multiplied human cells from the chamber;
- culturing the human cells in a nutrient medium for a period sufficient to accumulate a significant amount of hCG;
- and
- harvesting the accumulated hCG from the culture.
Dependencies are inferred from the claim text.