Process for the production of human luteinizing hormone
AssigneeKabushiki Kaisha Hayashibara Seibutsu Kagaku Kenkyujo
InventorKaname Sugimoto
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Abstract
The present invention relates to a process for the production of human luteinizing hormone (hLH). More precisely, the invention relates to a process for the mass production of hLH, comprising in vivo multiplication of human cells capable of producing hLH, using non-human warm-blooded animal, and exposure of the multiplied human cells to a luteinizing hormone inducer. The hLH production according to the invention is extremely higher, in terms of hLH production per cell, than that attained by conventional processes using in vitro tissue culture, thus, hLH can be used in a sufficient amount in the prevention and treatment of human diseases.
Claims9
A process for producing human luteinizing hormone (hLH), which comprises:
- (1) implanting human cells capable of producing hLH into a non-human warm-blooded animal;
- feeding the animal to cause the human cells therein to multiply;
- extracting and disaggregating the resultant multiplied human cells formed in the animal;
- culturing the human cells in a nutrient medium in the presence of a luteinizing hormone inducer for a period sufficient to accumulate a significant amount of hLH;
- and
- harvesting the accumulated hLH from the culture, or alternatively,
- (2) placing human cells capable of producing hLH in suspension in a diffusion chamber;
- embedding the chamber in or placing the chamber on a non-human warm-blooded animal in a manner such that the nutrient body fluid of the non-human warm-blooded animal is supplied to the cells within the chamber;
- feeding the animal to cause the human cells in the chamber to multiply;
- collecting the multiplied human cells from the chamber;
- culturing the human cells in a nutrient medium in the presence of a luteinizing hormone inducer for a period sufficient to accumulate a significant amount of hLH;
- and
- harvesting the accumulated hLH from the culture.
Dependencies are inferred from the claim text.