Process for the production of human follicle-stimulating hormone
AssigneeKabushiki Kaisha Hayashibara Seibutsu Kagaku Kenkyujo
InventorKaname Sugimoto
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Abstract
The present invention relates to a process for the production of human follicle-stimulating hormone (hFSH). More precisely, the invention relates to a process for the mass production of hFSH, comprising in vivo multiplication of human cells capable of producing hFSH, and exposure of the multiplied human cells to a follicle-stimulating hormone inducer. The hFSH production according to the invention is much higher than that attained by conventional processes using in vitro tissue culture; thus, hFSH can be used in a sufficient amount in the prevention and treatment of human diseases.
Claims10
A process for producing human follicle-stimulating hormone (hFSH) which comprises:
- (1) implanting human cells capable of producing hFSH into a non-human warm-blooded animal;
- feeding the animal to cause the human cells therein to multiply;
- extracting and disaggregating the resultant multiplied human cells formed in the animal;
- culturing the human cells in a nutrient medium in the presence of a follicle-stimulating hormone inducer for a period sufficient to accumulate a significant amount of hFSH;
- and
- harvesting the accumulated hFSH from the culture, or alternatively,
- (2) placing human cells capable of producing hFSH in suspension in a diffusion chamber;
- embedding the chamber in or placing the chamber, on a non-human warm-blooded animal in a manner such that the nutrient body fluid of the non-human warm-blooded animal is supplied to the cells within the chamber;
- feeding the animal to cause the human cells in the chamber to multiply;
- collecting the multiplied human cells from the chamber;
- culturing the human cells in a nutrient medium in the presence of a follicle stimulating hormone inducer for a period sufficient to accumulate a significant amount of hFSH;
- and
- harvesting the accumulated hFSH from the culture.
Dependencies are inferred from the claim text.