Reagent for the determination of .beta.-lactamase
AssigneeBoehringer Mannheim GmbH
InventorsPeter Stahl, Wolfgang Vomel, Hans Seidel
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Abstract
The present invention provides a process for the determination of .beta.-lactamases by the use of 7-cyanoacetylaminocephalosporanic acid and measurement of the colored material thereby formed, wherein ammonium ions are added to the material to be tested, together with phosphate ions and/or an agent splitting off oxygen. The present invention also provides a reagent for the determination of .beta.-lactamases based upon 7-cyanoacetylaminocephalosporanic acid, which reagent additionally contains ammonium ions, together with phosphate ions and/or an agent splitting off oxygen.
Claims20
Method for the determination of beta-lactamases comprising contacting the sample to be determined with 7-cyanoacetylaminocephalosporanic acid in an amount to form a quantitative color reaction and, in amounts effective to accelerate the color reaction, a synergistic mixture of ammonium ions and at least one member of the group consisting of phosphate ions and oxygen-splitting agents;
- and measuring the colored material formed as a measure of the beta-lactamases present.
Process for the determination of beta-lactamases which comprises contacting the sample to be determined with a combination of reagents consisting of 7-cyanoacetylaminocephalosporanic acid, ammonium ions, and at least one member of the group consisting of phosphate ions and oxygen-splitting agents, with a portion of the reagent combination being on the surface of a culture plate and with a barrier layer impermeable to the colored reaction product formed being provided in the culture surrounding said portion.
Reagent for the colorimetric determination of beta-lactamases by reaction with 7-cyanoacetylaminocephalosporanic acid, comprising:
- 7-cyanoacetylaminocephalosporanic acid, and in amounts effective to accelerate the color reaction, a synergistic mixture of ammonium ions, and
- at least one member of the group consisting of phosphate ions, and oxygen splitting agents.
Dependencies are inferred from the claim text.