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US 4,383,031 AGranted
Homogeneous chemiluminescent specific binding assay
AssigneeMiles Laboratories, Inc.
InventorsRobert C. Boguslaski, Robert J. Carrico
Life of this patent
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AssigneeMiles Laboratories, Inc.
InventorsRobert C. Boguslaski, Robert J. Carrico
Estimating the term…
A homogeneous specific binding assay which employs a substance having reactant activity, i.e., a reactant, in a chemiluminescent reaction as a labeling substance in the detection of a ligand in a liquid medium. The assay employs a conjugate formed of a specific binding substance coupled to the chemiluminescent reactant. The activity of the conjugated reactant as a constituent of the chemiluminescent reaction is affected by reaction between the specific binding substance in the conjugate and a specific binding counterpart thereto. The presence of a ligand in a liquid medium may be determined using competitive or displacement binding or sequential saturation techniques wherein the specific binding substance in the conjugate is the ligand or a specific binding analog thereof, or using a direct binding technique wherein the specific binding substance is a specific binding partner of the ligand. The effect of the specific binding reaction on the chemiluminescent activity of the conjugated reactant is related to the presence or amount of the ligand in the liquid medium tested.
A homogeneous specific binding assay method for determining a ligand in a liquid medium, comprising the steps of:
A homogeneous competitive binding assay method for determining a ligand in a liquid medium, comprising the steps of:
A homogeneous direct binding assay method for determining a ligand in a liquid medium, comprising the steps of:
A homogeneous method of determining the presence of a ligand in a medium suspected of containing said ligand which comprises;
Reagent means for use in a homogeneous specific binding assay for determining a ligand in a liquid medium, comprising (a) a conjugate comprising said ligand or an analog thereof labeled with a substance which is a reactant in a chemiluminescent reaction, and (b) a specific binding partner of said ligand, the ability of the chemiluminescent label to participate in said chemiluminescent reaction being changed when said conjugate is bound by said binding partner.
Dependencies are inferred from the claim text.