Stabilized Romanowsky stain solution
AssigneeMiles Laboratories, Inc.
InventorsJohn C. Liao, John L. Ponzo, Ellen M. Jenning
Life of this patent
Estimating the term…
Abstract
The present invention is a stabilized Romanowsky type stain solution. The stain solution which comprises azures, Methylene Blue and an eosin dye in methanol solution, is stabilized by the addition thereto of a stabilizing amount of a combination of dimethylamine hydrochloride and a second amine of the formula R.sub.2 NH.sub.2.sup.+ Cl.sup.- or R.sub.3 NH.sup.+ Cl.sup.- where R is ethyl, n-propyl or n-butyl.
Claims6
A Romanowsky-type stain comprising azures, methylene blue and an eosin dye in methanol solution which contains from 0.55 to 0.9 weight percent of a combination of dimethylamine hydrochloride and a second stabilizer which is a secondary or tertiary alkylamine hydrochloride of the formula R.sub.2 NH.sub.2.sup.+ Cl.sup.- or R.sub.3 NH.sup.+ Cl.sup.- where R is ethyl, n-propyl or n-butyl said dimethylamine hydrochloride and secondary or tertiary alkylamine hydrochloride being present in a ratio of dimethylamine hydrochloride to the other stabilizer in the range of from about 1:10 to 1:3.5.
A Romanowsky-type stain comprising 2 to 4 weight percent of a combination of azures, methylene blue and an eosin dye in methanol solution which contains from 0.55 to 0.9 weight percent of a combination of dimethylamine hydrochloride and diethylamine hydrochloride wherein the weight ratio of dimethylamine hydrochloride to diethylamine hydrochloride is in the range of from about 1:10 to 1:3.5.
Dependencies are inferred from the claim text.