Scintillation proximity assay

In an improved non-destructive method of biological fluid assay wherein antigens link antibody-coatings on fluid suspensions of both a plurality of tritiated-particulates and potentially scintillating-particulates within activating range one to the other, each of said scintillating particulates emitting detectable light-pulses upon activation responsive to bombardment of energy from each of said linked tritiated-particulates within said range, said detectable light-pulses initially measured correlatively to the amount of said antigens in said fluid, the improvement wherein of adding a known amount of said antibody-coated tritiated particulates and said antibody-coated scintillating particulates to said fluid, measuring the resulting detectable light pulses and correlating the reduction in measured light-pulses from said initially measured light-pulses to the reduction in the amount of antigens in said fluid linked to said two particulates, and this being further correlative to the amount of unbound antibodies initially available in said fluid.

In an improved non-destructive method of biological fluid assay wherein antibodies link antigen-coated tritiated-solid-phase materials to antigen-coated potentially scintillating-solid-phase materials within activating range one to the other, said scintillating-solid-phase materials being particulates and emitting detectable light-pulses upon activation responsive to bombardment of energy from said linked solid-phase-tritiated materials within said range, said detectable light-pulses initially measured correlatively to the amount of said antibody in said fluid, the improvement wherein of adding a known amount of said antigen-coated potentially scintillating particulates to said fluid, measuring the resulting detectable light-pulses and correlating the reduction in measured light-pulses from said initially measured light-pulses to the reduction in the amount of antibodies in said fluid linked to said two antigen-coated materials, and this being correlative to the amount of unbound antigens initially available in said fluid.

In an improved non-destructive method of biological fluid assay wherein antigens link antibody-coated tritiated solid-phase materials to antibody-coated potentially scintillating-solid-phase materials within activating range one to the other, said scintillating-solid-phase materials being particulates and emitting detectable light-pulses upon activation responsive to bombardment of energy from said linked tritiated-solid-phase-materials within said range, said detectable light-pulses initially measured correlatively to the amount of said antigens in said fluid, the improvement wherein of adding a known amount of said antibody-coated, potentially scintillating-particulates to said fluid, measuring the resulting detectable light-pulses and correlating the reduction in measured light-pulses from said initially measured light-pulses to the reduction in the amount of antigens in said fluid linked to said antibody-coated materials, and this being correlative to the amount of unbound antibodies initially available in said fluid.

In an improved non-destructive method of biological fluid assay wherein antibodies link antigen-coated tritiated-solid-phase materials to antigen-coated potentially scintillating-solid-phase materials within activating rante one to the other, said tritiated-solid-phase materials being particulate and said scintillating solid-phase-materials emitting detectable light-pulses upon activation responsive to bombardment of energy from said linked tritiated-particulates within said range, said detectable light-pulses initially measured correlatively to the amount of said antibody in said fluid, the improvement wherein of adding a known amount of said antigen-coated tritiated-particulates to said fluid, measuring the resulting detectable light-pulses and correlating the reduction in measured light-pulses from said initially measured light-pulses to the reduction in the amount of antibodies in said fluid linked to said two antigen-coated materials, and this being correlative to the amount of unbound antigens initially available in said fluid.

In an improved non-destructive method of biological fluid assay wherein antigens link antibody-coated tritiated-solid-phase materials to antibody-coated potentially scintillating-solid-phase materials within activating rante one to the other, said tritiated-solid-phase materials being particulates and said scintillating solid-phase materials emitting detectable light-pulses upon activation responsive to bombardment of energy from said linked tritiated-particules within said range, said detectable light-pulses initially measured correlatively to the amount of antigens in said fluid, the improvement wherein of adding a known amount of said antibody-coated tritiated-particulates to said fluid, measuring the resulting detectable light-pulses and correlating the reduction in measured light-pulses from said initially measured light-pulses to the reduction in the amount of antigens in said fluid linked to said two antibody-coated materials, and this correlative to the amount of unbound antibodies initially available in said fluid.