Determination of antibacterial agents
AssigneesTeva Pharmaceutical Industries Ltd., Yissum Research Development Company
InventorNathan Citri
Life of this patent
Estimating the term…
Abstract
The presence of .beta.-lactam antibiotics in test material such as food, infusions, vaccines, blood for transfusion, body fluids, etc. may be determined by: seeding a nutrient medium with a .beta.-lactamase generating bacterium or spores thereof; applying a sample of said test material to a site on the so-called nutrient medium; then incubating the medium under conditions inducive to the generation of .beta.-lactamase by said bacteria; and assaying the .beta.-lactamase thus produced.
Claims10
A method for the simultaneous determination of a .beta.-lactam antibiotic (BLA) and a non-BLA antibacterial agent in a test material, comprising:
- (i) seeding a nutrient medium with a strain of .beta.-lactamase generating bacterium or spores thereof;
- (ii) applying to the seeded nutrient medium at two discrete sites two samples of the test material, one of them together with a BLA;
- (iii) then incubating the nutrient medium under conditions inducive to the generation of .beta.-lactamase by said bacteria;
- (iv) assaying said discrete sites for .beta.-lactamase (development);
- and
- (v) comparing the development of said two sites.
Dependencies are inferred from the claim text.