# US 4,380,583 A · Method of preparing seeding material for production of citric acid

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- Number: US 4,380,583 A
- Document ID: US4380583A
- Status: Granted
- Kind: A
- Application number: 06/252,040
- Filed: 1981-04-08
- Granted: 1983-04-19
- Earliest claimed: 1980-04-19
- Inventors: Roman Y. Karklin; Alma A. Rumba; Via K. Azanda
- Web page: https://patentgrep.com/patent/US4380583A

## CPC

- C12N 3/00: Spore forming or isolating processes
- C12P 7/48: Tricarboxylic acids, e.g. citric acid
- C12N: MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA (microbiological testing media C12Q1/00)
- C12P: FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE {(brewing of beer C12C; producing vinegar C12J; producing specific peptides or proteins C07K; producing enzymes C12N9/00; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification C12N15/00; measuring or testing processes involving enzymes or microorganisms C12Q; measuring or testing processes involving nucleic acid amplification reactions C12Q1/6844; fermentation processes to form a food composition, A21 or A23; compounds in general, see the relevant compound class, e.g. C01, C07)}

## Abstract

Disclosed is a method of preparing a seeding material for the production of citric acid by cultivating spores of a fungus on a nutrient medium containing carbon and nitrogen sources and mineral salts and by separating spores from the nutrient medium. The fungus used is the strain Aspergillus niger R-3 selected from the strain Aspergillus niger -119 by the stepwise action on it of ethyleneimine, N-nitrosomethylurea and ultraviolet radiation.

## Claims

### Claim 1

A method of preparing a seeding material for the production of citric acid comprising cultivating the fungus Aspergillus niger R-3 strain TsMPMF 132 on a nutrient medium containing a carbon source selected from the group consisting of malt extract and beer must, a nitrogen source, and at least one mineral salt to form spores and separating said spores in an amount in excess of 1.3 g/dm from the nutrient medium.

### Claim 2 (depends on 1)

The method of claim 1 further comprising conducting the cultivating step at a temperature between 20.degree. and 34.degree. C.

### Claim 3 (depends on 1, 2)

The method of claim 1 or 2 wherein said nitrogen source is selected from urea, ammonium nitrate and ammonium chloride.