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US 4,380,582 AGranted
Preparation of dry variola virus
AssigneeThe United States of America as represented by the Secretary of the Army
InventorsMichael D. Orlando, Jean M. Riley
Life of this patent
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Abstract
A method of preparing a variola virus which comprises growing the virus on the embryos of chicken eggs, recoverng the inoculated embryos, stabilizing the recovered embryos with a mixture comprising; lactose, raffinose, lysine, sodium glutamate, dextrin, isoniazid and thiourea.
Claims1
In a process for the preparation of a variola virus which comprises the steps of:
- chorioallantoic inoculation of the embryos of fertile chicken eggs, incubating the eggs for a period of time sufficient to cultivate the virus, separating the embryos from the rest of the eggs, stabilizing the embryos, homogenizing, differential centrifuging the resultant slurry, freezing the slurry into pellets and drying the pellets under a vacuum;
- the improvement wherein the stabilizing comprises the addition of:
- 2.5% lactose, 2.5% raffinose, 1.5% lysine, 1.5% sodium glutamate, 1.5% dextrin, 0.5% isoniazid, and 0.5% thiourea, per volume of embryos.
Dependencies are inferred from the claim text.