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US 4,380,511 AGranted
Purification of bovine thrombin
AssigneeMiles Laboratories, Inc.
InventorsFrank J. Mannuzza, Joseph G. Montalto
Life of this patent
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Abstract
A method for removing pyrogenic material from bovine thrombin is disclosed. The method involves forming a complex of a blue dye and a polysaccharide and equilibrating the complex with a low ionic strength salt solution. Bovine thrombin is added to the complex. The pyrogenic material is removed from the bovine thrombin by washing the bovine thrombin with a low ionic strength salt solution, and the pyrogen-free bovine thrombin is recovered.
Claims6
A method for removing pyrogenic material from pyrogen-containing bovine thrombin which comprises the steps of forming a complex of a dye having the structure:
- ##STR3## where R.sub.1 and R.sub.2 are hydrogen or SO.sub.3 H, and R.sub.3 is Cl or O;
- and a dextran which has been cross-linked with epichlorohydrin to produce a three-dimensional network of polysaccharides, equilibrating the complex with a low ionic strength salt solution, contacting said complex with said pyrogen-containing bovine thrombin, washing said thrombin with a low ionic strength salt solution to remove said pyrogens, and recovering pyrogen-free bovine thrombin.
Dependencies are inferred from the claim text.