Hemolytic method for the kinetic determination of antistreptolysin O antibodies in blood or serum samples, using oxidized streptolysin O
AssigneeDiesse Diagnostica Senese S.r.l.
InventorAntonio Ricci
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Abstract
The hemolytic method for the kinetic determination of antistreptolysin O antibodies (ASO) in blood samples consists of reacting a first reagent containing a single dose of oxidized SO with the specific antibodies which may be present in the blood sample under examination, allowing the necessary time to pass for the reaction between the oxidized SO and said antibodies to take place, returning the oxidized SO to its reduced state by adding a second reagent, and measuring the rate of hemolysis. The kinetic determination of the ASO titre is obtained by comparing said rate of hemolysis with the rate of hemolysis shown graphically for samples of known ASO titre.
Claims16
A hemolytic method for the kinetic determination of antistreptolysin O antibodies (ASO) in a whole blood sample, consisting of:
- reacting a first reagent containing a single dose of oxidized streptolysin O (SO) with the specific antibodies which may be present in the blood sample under examination;
- allowing the necessary time to pass for the reaction between the oxidized SO and said antibodies to take place;
- returning the oxidized SO to its reduced state by adding a second reagent;
- measuring the rate of hemolysis;
- and
- comparing said rate of hemolysis with the rate of hemolysis shown graphically for samples of known ASO titre, thus obtaining the kinetic determination of the ASO titre of the blood sample under examination.
Dependencies are inferred from the claim text.