Immobilization of polynucleotides and polypeptides with tritylated polysaccharides

A composition of matter comprising a poly A containing ribonucleic acid attracted to a substantially hydrated polysaccharide through a triphenylmethyl ether group bonded to said polysaccharide.

A composition of matter, comprising a polypeptide attracted to a substantially hydrated polysaccharide through a triphenylmethyl ether group bonded to said polysaccharide, said polysaccharide being hydrated at least about 60 percent by weight.

A composition of matter, comprising a polynucleotide or polypeptide attracted to a substantially hydrated polysaccharide through a triphenylmethyl ether group bonded to said polysaccharide, said polysaccharide being a member selected from the group consisting of agarose, cellulose, dextran, glycogen, carragheenan, starch, or a mixture thereof.

A process for the immobilization of a polynucleotide or polypeptide, comprising forming a reaction product of triphenylmethyl ether of polysaccharide, hydrating substantially said reaction product, and reacting said hydrated reaction product with said polynucleotide or polypeptide to produce an immobilized composition of matter thereof.

A process for the immobilization of a poly A containing ribonucleic acid, comprising forming a reaction product of triphenylmethyl ether of polysaccharide, hydrating substantially said reaction product, and reacting said hydrated reaction product with a poly A containing ribonucleic acid to immobilize said acid.

A process for immobilizing a polypeptide, comprising forming a reaction product of triphenylmethyl ether of polysaccharide, hydrating substantially said reaction product, and reacting said hydrated reaction product with polypeptide to immobilize the same.

A process for immobilization of a polynucleotide or polypeptide, comprising forming a reaction product of triphenylmethyl ether of polysaccharide, said polysaccharide being a member selected from the group consisting of agarose, cellulose, dextran, glycogen, carragheenan, starch, or a mixture thereof, hydrating substantially said reaction product, reacting said product with a polynucleotide or polypeptide to produce an immobilized composition of matter thereof.

A process of isolating a polynucleotide or polypeptide, comprising contacting a composition including said polynucleotide or polypeptide with a substantially hydrated gel of triphenylmethyl ether of a polysaccharide.