Process for the manufacture of 1.alpha.-hydroxydehydroepiandrosterone
AssigneeHoffmann-La Roche Inc.
InventorsAkiko Fujiwara, Chikara Miyamoto, Toru Okuda
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Abstract
The invention relates to a process for producing 1.alpha.-hydroxydehydroepiandrosterone by fermenting or reacting dehydroepiandrosterone or a derivative thereof with microorganisms of the species Penicillium oxalicum or Aspergillus terreus or mycelium or extracts therefrom obtained when the microorganisms are in a stationary phase of growth. The invention process produces steroid compounds which are pharmacologically valuable substances.
Claims9
A process for producing 1-.alpha.-hydroxydehydroepiandrosterone comprising adding dehydroepiandrosterone or 3-acyldehydroepiandrosterone as a substrate to microorganisms of the species Penicillium oxalicum or Aspergillus terreus fermenting in a culture medium, the addition of substrate being made at a time when the microoganisms are in a stationary phase of growth and for a time sufficient for conversion of the substrate.
A process for producing 1-.alpha.-hydroxy dehydroepiandrosterone comprising reacting dehydroepiandrosterone or 3-acyl-dehydroepiandrosterone as a substrate with mycelium isolated from a culture of microorganisms of the species Penicillium oxalicum or Aspegillus terreus, the isolation being made at a time when the microorganisms are in a stationary phase of growth and the substrate reacting for a time sufficient for conversion of the substrate.
A process for producing 1-.alpha.-dehydroxyepiandrosterone comprising reacting dehydroepiandrosterone or 3-acyl-dehydroepiandrosterone as a substrate to microorganisms of the species Penicillium oxalicum or Aspergillus terreus fermenting in a culture medium, said substrate being in a concentration of 1-15 grams per liter of medium and being added at a time when the microorganisms are in a stationary phase of growth and for a time sufficient for conversion of the substrate.
A process according to claim 8 wherein the microorganism is Penicillium Oxalicum IFO-5748 (FERM-P No. 4727), Penicillium oxalicum IFO-6579 (FERM-P No. 4728), Penicillium oxalicum ISO-7000 (FERM-P No. 4729), Penicillium oxalicum IFO-7085 (FERM-P No. 4730) or Aspergillus terreus IFO-6123 (FERM-P No. 4726).
Dependencies are inferred from the claim text.