Quantitative analysis of uric acid
AssigneeC. H. Boehringer Sohn
InventorsGunther Gorka, Klaus Stinshoff
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Abstract
This invention relates to a process for determining the uric acid content of biological material by contacting the biological material with a reagent composition comprised of uricase, catalase, aldehyde dehydrogenase, a lower alkanol, and nicotinamide adenine dinucleotide or nicotinamide adenine dinucleotied phosphate, the improvement which comprises determining the uric acid content in the presence of 2-mercaptosuccinic acid.
Claims6
In a process for determining the uric acid content of biological material by contacting the biological material with a reagent composition containing uricase, catalase, aldehyde dehydrogenase, a lower alkanol, and nicotinamide adenine dinucleotide or nicotinamide adenine dinucleotide phosphate,
- the improvement which comprises contacting the biological material with a reagent composition comprising:
- (a) from about 25 to 500 IU/liter of uricase;
- (b) from about 300 to 1000 kIU/liter of catalase;
- (c) from about 100 to 500 IU/liter of aldehyde dehydrogenase;
- (d) from about 0.5 to 2 mol/liter of ethanol;
- (e) from about 0.2 to 1.5 mmol/liter of NAD.sup.+ or NADP.sup.+ ;
- (f) from about 20 to 100 mmol/liter of buffer;
- and
- (g) from about 0.0001 to 0.1 mol/liter of 2-mercaptosuccinic acid.
A reagent composition for determining the content of uric acid in biological material which comprises:
- (a) from about 25 to 500 IU/liter of uricase;
- (b) from about 300 to 1000 kIU/liter of catalase;
- (c) from about 100 to 500 IU/liter of aldehyde dehydrogenase;
- (d) from about 0.5 to 2 mol/liter of ethanol;
- (e) from about 0.2 to 1.5 mmol/liter of NAD.sup.+ or NADP.sup.+ ;
- (f) from about 20 to 100 mmol/liter of buffer;
- and
- (g) from about 0.0001 to 0.1 mol/liter of 2-mercaptosuccinic acid.
Dependencies are inferred from the claim text.