17 .alpha.-Dihydroequilin hapten and assay method
AssigneeAmerican Home Products Corporation
InventorPemmaraju N. Rao
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Abstract
A hapten is obtained by replacing the 3-hydroxy group of 17 .alpha.-dihydroequilin with HO--CO--A--O-- wherein A is an alkylene of one to six carbon atoms. The hapten is conjugated with an immunological carrier to provide an immunogen, which in turn produces a specific antiserum to 17 .alpha.-dihydroequilin. The antiserum is used in a radioimmunoassay for 17 .alpha.-dihydroequilin.
Claims10
A compound of the formula ##STR3## wherein A is an alkylene group of one to six carbon atoms.
An immunogen comprising 1,3,5(10),7-estratetraene-3,17.alpha.-diol 3O-carboxymethyl ether conjugated to an immunological carrier.
In a radioimmunoassay procedure of 1,3,5(10),7-estratetraene-3,17.alpha.-diol, wherein the sample for assay contains 1,3,5(10),7-estratetraene-3,17.alpha.-diol in the form of its 3-sulfate salt, the improvement which comprises subjecting the sample to hydrolysis conditions in the presence of a water soluble, non-protein binding antioxidant which does not interfere with the radioimmunoassay procedure.
In a radioimmunoassay procedure of 1,3,5(10),7-estratetraene-3,17.alpha.-diol, wherein the sample for assay contains 1,3,5(10),7-estratetraene-3,17.alpha.-diol in the form of its 3-sulfate salt, the improvement which comprises subjecting the sample to hydrolysis conditions in the presence of ascorbic acid.
Dependencies are inferred from the claim text.