Polysaccharide S-60 and bacterial fermentation process for its preparation
AssigneeMerck & Co., Inc.
InventorsKenneth S. Kang, George T. Veeder
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Abstract
A novel polysaccharide S-60 is disclosed composed of principally carbohydrate, 10-15% protein, and 3-4.5% acetyl groups as the O-glycosidically linked ester. The carbohydrate portion contains about 11% glucuronic acid (based on wt. gum) and the neutral sugars rhamnose and glucose, the latter having an approximate molar ratio of 3 to 2. This polysaccharide is produced by a new Pseudomonas species, P. elodea, in a suitable fermentation medium.
Claims4
A process for producing heteropolysaccharide S-60 comprising incubating the organism Pseudomonas elodea in a fermentation medium containing a carbon source, a source of potassium ions, a source of nitrogen, trace inorganic elements, and water at a temperature of 28.degree.-32.degree. C. and pH of 6-8 for 40-60 hours, and recovering the heteropolysaccharide by precipitation with a suitable lower alcohol.
The biologically pure culture culture of Pseudomonas elodea, having the identifying characteristics of ATCC 31461 said culture being capable of producing heteropolysaccharide S-60 in recoverable amounts by fermentation in an aqueous nutrient medium containing a carbon source.
Dependencies are inferred from the claim text.