Mobile phase for liquid chromatography
AssigneeThe Salk Institute for Biological Studies
InventorJean E. F. Rivier
Life of this patent
Estimating the term…
Abstract
A mobile phase for use in reversed phase high performance liquid chromatography. The mobile phase is a mixture of a polar organic solvent selected from acetonitrile and methanol and an aqueous solution of a salt of a tertiary amine and phosphoric acid or formic acid. The tertiary amines are selected from the group consisting of trialkyl amines and cyclic tertiary amines such as N-methyl morpholine, wherein the alkyl moiety is selected from methyl, ethyl, propyl, butyl and mixtures thereof.
Claims13
A mobile phase for use in analysis of peptides by reversed-phase high performance liquid chromatography comprising a mixture of a polar organic solvent selected from the group consisting of acetonitrile and methanol and an aqueous solution of a tertiary amine selected from the group consisting of trialkylammonium phosphate, trialkylammonium formate and N-alkyl morpholine, wherein said alkyl moiety is selected from the group consisting of methyl, ethyl, propyl, butyl and mixtures thereof.
A method for resolving mixtures of peptides by reversed-phase high performance liquid chromatography comprising providing a column packed with hydrocarbonaceous functional groups bonded to a carrier, introducing a peptide mixture into the end of said column and eluting said column with a mobile phase comprising a mixture of a polar solvent selected from the group consisting of acetonitrile and methanol and an aqueous solution of a tertiary amine selected from the group consisting of trialkylammonium phosphate, trialkylammonium formate and N-alkyl morpholine wherein said alkyl moiety is selected from methyl, ethyl, propyl, butyl and mixtures thereof.
Dependencies are inferred from the claim text.