Process for producing glucose/fructose syrups from unrefined starch hydrolysates

A process for producing a glucose/fructose syrup comprising liquefying starch at a pH of less than about 6.0 and enzymatically saccharifying the liquefied starch under controlled conditions to provide an unrefined glucose-containing hydrolysate having present not more than about 100 ppm of calcium ions based on dry substance starch and wherein the mole ratio of non-enzymatically generated ketose sugars is less than about 2 (moles per 100 moles of hexose units), treating the hydrolysate to remove non-starch material therefrom, and contacting the unrefined hydrolysate with immobilized glucose isomerase to convert at least a portion of the glucose to fructose.