Method of increasing the antibiotic yield of producing organisms
AssigneeWisconsin Alumni Research Foundation
InventorBernard Weisblum
Life of this patent
Estimating the term…
Abstract
A method of increasing the yield of a desired product, such as an antibiotic, by an organism which normally must first become inducibly resistant to that product before it can produce the product in maximum yields comprises producing constitutively resistant cells of the organism by supplementing a culture of the organism with an agent in which only cells able to specifically modify the 23S ribosomal RNA constitutively, rather than inducibly, survive thereby producing an organism in which the resistance to the product is expressed without the need for activation by the induction process; followed by purification and utilization of that organism for increased product production.
Claims10
A method of increasing the yield of a desired product by an organism selected from Streptomyces, Micromonospora and Nocardia which organism produces that product but which normally must first become inducibly resistant to that product before it can produce the product in maximum yields, which method comprises producing constitutively resistant cells of the organism by supplementing a culture of the organism with an agent in which only cells able to specifically modify the 23S ribosomal RNA constitutively, rather than inducibly, survive thereby producing an organism in which the resistance to the product is expressed without the need for activation by the induction process;
- followed by purification and utilization of that organism for increased production.
A method of increasing the antibiotic yield of an antibiotic producing organism which normally must first become inducibly resistant to the antibiotic which it produces before it can produce the antibiotic in maximum yields, which method comprises producing constitutively resistant cells of the organism by supplementing a culture of the organism with an agent in which only cells able to specifically modify the 23S ribosomal RNA constitutively, rather than inducibly, survive thereby producing an organism in which the resistance to the antibiotic is expressed without the need for activation by the induction process;
- followed by purification and utilization of that organism that increased antibiotic production.
Dependencies are inferred from the claim text.