Antibiotic substances and processes for production thereof
AssigneeMeiji Seika Kaisha, Ltd.
InventorsNorio Ezaki, Takashi Shomura, Tomizo Niwa, Michio Kojima, Shigeharu Inouye, Tatsuo Ito
Life of this patent
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Abstract
Antibiotics, designated as SF-2080A substance and SF-2080B substance, are described, and are obtained by cultivating an SF-2080 substance-producing strain belonging to the genus Streptomyces, for example, Streptomyces sp. SF-2080 (FERM-P No. 5072, ATCC No. 31673), in a nutrient medium, and recovering the desired substance from the culture.
Claims2
An SF-2080A substance having the following properties:
- Elemental Analysis (by weight):
- C, 27.06%;
- H, 1.17%;
- N, 14.84%;
- Cl, 39.23%;
- Molecular Weight:
- 181 (according to mass spectrum);
- Molecular Formula:
- C.sub.4 H.sub.2 N.sub.2 O.sub.2 Cl.sub.2 ;
- Melting Point:
- 205.degree. to 207.degree. C. (with decomposition);
- Optical Rotation:
- [.alpha]sub.D.sup.25 =0 (C=0.5, methanol);
- Ultraviolet Absorption Spectrum:
- as shown in FIG. 1:
- The absorption maximums are 269 and 320 nm in a methanol solution, 271 and 320 nm in a 0.01-N hydrochloric acid methanol solution, and 220 and 325 nm in a 0.01-N sodium hydroxide methanol solution;
- Infrared Absorption Spectrum:
- as shown in FIG. 2:
- The absorption bands, as determined using the potassium bromide tablet method, are 3270, 3150, 1570, 1510, 1480, 1400, 1360, 1280, 1200, 1140, 1070, 960, 830, 820, 750 cm.sup.-1 ;
- Color Reactions:
- positive for the iodine reaction and Lemieux reaction, and negative for the ninhydrin reaction and ferric chloride reaction;
- Appearance and Color:
- light brown to yellow needle-shaped crystals;
- Electrical Properties:
- behaves as neutral substance on High Voltage Paper Electrophoresis;
- Rf Values on Thin Layer Chromatography (silica gel):
- 0.68 (benzene/acetone=2:1 by volume), 0.73 (chloroform/methanol=10:1 by volume);
- Solubility:
- easily soluble in methanol and acetone, soluble in chloroform, and sparingly soluble in water and n-hexane;
- and ##STR5##
An antimycotic agent containing a carrier and an antimycotically effective amount of an active component SF-2080A substance represented by the following formula:
- ##STR6##
Dependencies are inferred from the claim text.