Biologically pure culture of Actinoplanes missouriensis
AssigneeEli Lilly and Company
InventorsManuel Debono, Kurt E. Merkel, Robert E. Weeks, Herald J. Cole
Life of this patent
Estimating the term…
Abstract
Antibiotic A-4696 factors B.sub.1, B.sub.2, B.sub.3, C.sub.1a, C.sub.3, and E.sub.1 are isolated from antibiotic A-4696, the latter being produced by Actinoplanes missouriensis strains ATCC 31680, ATCC 31682, and ATCC 31683 under submerged aerobic conditions in a culture medium and isolated from the fermentation broth by absorption on an ion exchange resin and eluted therefrom at p.sup.H 10.5 with sodium hydroxide. The novel factors are separated by thin layer chromatography and high pressure liquid chromatography and have antimicrobial and growth promotant activity.
Claims2
The biologically pure culture of Actinoplanes missouriensis strain ATCC 31683 which when cultured in a nutrient medium under submerged aerobic fermentation conditions, produces antibiotic A-4696 for the subsequent isolation of antibiotic A-4696 factors B.sub.1 and C.sub.1a.
The biologically pure culture of Actinoplanes missouriensis strain ATCC 31683 which when cultured in a nutrient medium under submerged aerobic fermentation conditions, produces antibiotic A-4696 for the subsequent isolation of antibiotic A-4696 factors B.sub.3 and E.sub.1.
Dependencies are inferred from the claim text.