Selective medium composition and method for the detection of Actinomyces viscosus and Actinomyces naeslundii
AssigneeForsyth Dental Infirmary for Children
InventorHarold V. Jordan
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Abstract
A selective medium composition for the growth and detection of Actinomyces viscosus or Actinomyces naeslundii, which composition comprises: a solid medium selective to induce the growth of Actinomyces viscosus or Actinomyces naeslundii; a nutrient agent to induce the substantial growth of Actinomyces viscosus or Actinomyces naeslundii; a cadmium compound; a fluoride compound; a flavine compound; and the cadmium compound, the fluoride compound and the flavine compound all present in a concentration sufficient to inhibit the substantially full growth of interfering microorganisms, but in a concentration insufficient to inhibit the substantial growth of Actinomyces viscosus or Actinomyces naeslundii.
Claims29
A selective medium composition for the growth and detection of Actinomyces viscosus or Actinomyces naeslundii, which composition comprises:
- (a) a solid medium selective to induce the growth of Actinomyces viscosus or Actinomyces naeslundii;
- (b) a nutrient agent to induce the substantial growth of Actinomyces viscosus or Actinomyces naeslundii;
- (c) a cadmium compound;
- (d) a fluoride compound;
- (e) a flavine compound;
- and
- (f) the cadmium compound, the fluoride compound and the flavine compound all present in a concentration sufficient to inhibit the substantially full growth of interfering microorganisms, but in a concentration insufficient to inhibit the substantial growth of Actinomyces viscosus or Actinomyces naeslundii.
A selective medium composition for the growth and detection of Actinomyces viscosus or Actinomyces naeslundii, which composition comprises:
- ______________________________________ Per Liter ______________________________________ Trypticase Soy broth 10 gm to 50 gm glucose 1 gm to 10 gm agar 10 gm to 50 gm cadmium sulfate 10 mg to 13 mg sodium or potassium fluoride 80 mg to 250 mg neutral acriflavine 1.125 mg to 2.50 mg sodium or potassium tellurite 1.0 mg to 5.0 mg basic fuchsin 0.1 mg to 1.0 mg animal blood 10 ml to 100 ml ______________________________________
A selective medium composition for the growth and detection of Actinomyces viscosus or Actinomyces naeslundii, which composition comprises:
- ______________________________________ Per Liter ______________________________________ Trypticase Soy broth about 30 gm glucose about 5 gm agar about 15 gm cadmium sulfate about 13 mg sodium fluoride about 80 mg neutral acriflavine about 1.20 mg potassium tellurite about 2.50 mg basic fuchsin about 0.25 mg defibrinated sheep blood about 50 ml ______________________________________
A method for the selected growth of Actinomyces viscosus or Actinomyces naeslundii, which method comprises:
- (a) providing a selective medium composition for the growth and detection of Actinomyces viscosus or Actinomyces naeslundii, which composition comprises
- (i) a solid medium selective to induce the growth of Actinomyces viscosus or Actinomyces naeslundii,
- (ii) a nutrient agent to induce the substantial growth of Actinomyces viscosus or Actinomyces naeslundii,
- (iii) a cadmium compound,
- (iv) a fluoride compound,
- (v) a flavine compound, and
- (vi) the cadmium compound, the fluoride compound and the flavine compound all present in a concentration sufficient to inhibit the substantially full growth of interfering microorganisms, but in a concentration insufficient to inhibit the substantial growth of Actinomyces viscosus or Actinomyces naeslundii;
- (b) inoculating the medium with a selected microorganism;
- and
- (c) culturing the microorganism Actinomyces viscosus or Actinomyces naeslundii in the medium under aerobic conditions.
A selective medium composition for the growth and detection of Actinomyces viscosus or Actinomyces naeslundii, which composition comprises:
- (a) a solid medium selective to induce the growth of Actinomyces viscosus or Actinomyces naeslundii;
- (b) a nutrient agent to induce the substantial growth of Actinomyces viscosus or Actinomyces naeslundii;
- (c) an acid salt cadmium compound in concentration of from about 2 to 50 mg per liter of the composition;
- (d) an alkali metal salt fluoride compound in a concentration of from about 30 to 250 mg per liter of the composition;
- (e) an acriflavine compound in a concentration of from about 0.5 to 5.0 mg per liter of the composition;
- (f) the cadmium compound, the fluoride compound and the flavine compound all present in a concentration sufficient to inhibit the substantially full growth of interfering microorganisms, but in a concentration insufficient to inhibit the substantial growth of Actinomyces viscosus or Actinomyces naeslundii.
Dependencies are inferred from the claim text.