Back to results
US 4,375,163 AGranted
Method and apparatus for on-column detection in liquid chromatography
AssigneeVarian Associates, Inc.
InventorFrank J. Yang
Life of this patent
Estimating the term…
Abstract
On-column detection by an optical detector is accomplished with a flexible silica column. The flexible fused silica column has an inner diameter less than 500 .mu.m, an external protective coating with a stripped portion near the end of the column. The stripped portion is placed in the working path of an optical detector in order to detect and resolve the sample.
Claims10
A method for high resolution on-column detection in high resolution liquid chromatography, comprising:
- providing a fused silica microcapillary column having an inner diameter less than 500 .mu.m;
- activating the interior of said column with a chromatographically active material;
- applying a protective coating to the exterior of said column;
- removing a segment of said protective coating to expose the underlying segment of said fused silica column;
- providing an optical chromatographic detector;
- positioning said exposed segment of said column in the optical path of said detector;
- flowing a sample containing species to be identified through said column;
- and
- detecting separated species as they flow through said exposed segment of said column.
6 more elements
Apparatus for on-column detection in high resolution liquid chromatography comprising:
- a flexible fused silica column having an inner diameter less than 500 .mu.m, said column being chromatographically activated and also having an external protective coating, said external coating having a segment exposed along the end of its length;
- and
- an optical chromatographic detector configured to receive said column so that said exposed segment lies in the optical detection path of said detector.
Dependencies are inferred from the claim text.